Ian Garrard

Research Fellow Dr Ian Garrard

Contact

Heinz Wolff 001
Brunel University London
Uxbridge
UB8 3PH
United Kingdom
Tel: +44 (0)1895 266908
Email: ian.garrard@brunel.ac.uk

About Ian

Dr Ian Garrard is Manager of the Advanced Bioprocessing Centre at Brunel Institute for Bioengineering. With a team of around 12 people, and research projects funded by both the UK Research Councils and the bioseparations industry, the Advanced Bioprocesing Centre specialises in applications of liquid-liquid chromatography and has grown substantially in size and reputation since its official launch in April 2005.

Dr Garrard came to the University from a strong industrial background, having worked as a Senior Downstream Processing Chemist for Pfizer Ltd and as a Natural Products Isolation Scientist for the research company Xenova Ltd. He began his academic career when he joined Brunel University in September 2002.

With a PhD in the development of bioprocessing protocols for industrial application, Dr Garrard is keen to interface the high quality research at universities with the industrial demand for quick results and simple protocols. Current research interests include the purification of Traditional Chinese Medicines, the separation of proteins and monoclonal antibodies, the use of liquid-liquid systems as a bioreactor/separator, and the rapid scale-up of liquid-liquid chromatography to manufacturing levels.

Research Area

Bioprocess Engineering

Publications

Journal Papers

(Forthcoming) Garrard, I., Fernando, S., Grudzien, L. and Fisher, D., Use of robotics for two phase solvent system selection in countercurrent chromatography, Journal of Chromatography A forthcoming

(2013) Costa, FDN., Garrard, I., Da Silva, AJR. and Leitão, GG., Changes in the mobile phase composition on a stepwise counter-current chromatography elution for the isolation of flavonoids from Siparuna glycycarpa, Journal of Separation Science 36 (14) : 2253- 2259

(2012) de Lima e Silva, TA., Pereira Bresolin, IRA., de Campos Takaki, GM., Aoyma, H., Garrard, I. and Tambourgi, EB., Study of enzyme activity and stability to pH and temperature of bromelain extracted from curaua (Ananas erectifolius) purple and white., Int.Review of Chem.Eng (I.R.E.Ch.E)

(2012) Skalicka-Wozniak, K., Mroczek, T., Garrard, I. and Glowniak, K., Isolation of the minor and rare constituents from fruits of Peucedanum alsaticum L using high-performance countercurrent chromatography ., J.Sep.Sci (35) : 790- 797

(2012) Skalicka-Woźniak, K., Mroczek, T., Garrard, I. and Glowniak, K., Isolation of the minor and rare constituents from fruits of Peucedanum alsaticumL. using high-performance counter-current chromatography, Journal of Separation Science 35 (7) : 790- 797

(Forthcoming) Yeh, JC., Cho, CW., Garrard, IJ. and Glowniak, K., Bioactivity guided fractionation of the volatile oil of Angelica sinensis using countercurrent chromatography, gas chromatography and the MTS bioassay, Journal of Chromatography A forthcoming

(2012) Yeh, J-C., Garrard, IJ., Cho, CWC., Annie Bligh, SW., Lu, G-H., Fan, T-P. and Fisher, D., Bioactivity-guided fractionation of the volatile oil of Angelica sinensis radix designed to preserve the synergistic effects of the mixture followed by identification of the active principles, Journal of Chromatography A 1236 132- 138

(2009) Skalicka-Woźniak, K., Mroczek, T., Garrard, I. and Glowniak, K., Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography, Journal of Chromatography A 1216 (30) : 5669- 5675

(2009) Skalica-Woźniak, K., Mroczek, T., Garrard, I. and Glowniak, K., Isolation and purification of new minor dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography (Abstract), Planta Medica 75 (9) : 1057-

(2008) Garrard, IJ., Fisher, D. and Sutherland, IA., Dynamic extraction: a high-speed, high-capacity purification process that is rapidly scalable, LC/GC North America 26 (5) : 2- 7

(2008) Sutherland, IA., Audo, G., Bourton, E., Couillard, F., Fisher, D., Garrard, I., Hewitson, P. and Intes, O., Rapid linear scale-up of a protein separation by centrifugal partition chromatography, Journal of Chromatography A 1190 (1-2) : 57- 62

(2007) Fisher, D., Garrard, IJ., van den Heuvel, R., Sutherland, IA., Chou, FE. and Fahey, JW., Errata: Technology transfer and scale up of a potential cancer-preventative plant secondary metabolite-glucoraphanin (vol 28, pg 1913, 2005), Journal of Liquid Chromatography & Related Technologies 30 (17) : 2649-

(2007) Chen, L., Zhang, Q., Yang, G., Fan, L., Tang, J., Garrard, I., Ignatova, S., Fisher, D. and Sutherland, IA., Erratum to: "Rapid purification and scale-up of honokiol and magnolol using high-capacity high-speed counter-current chromatography"[J. Chromatogr. A 1142 (2007) 115–122], Journal of Chromatography A 1163 (1-2) : 337- 337

(2007) Wade, KL., Garrard, IJ. and Fahey, JW., Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates, Journal of Chromatography A 1154 (1-2) : 469- 472

(2007) Wood, P., Ignatova, S., Janaway, L., Keay, D., Hawes, D., Garrard, I. and Sutherland, IA., Counter-current chromatography separation scaled up from an analytical column to a production column, Journal of Chromatography A 1151 (1-2) : 25- 30

(2007) Chen, LJ., Zhang, Q., Yang, GL., Fan, LY., Tang, J., Garrard, I., Ignatova, S., Fisher, D. and Sutherland, IA., Rapid purification and scale-up of honokiol and magnolol using high-capacity high-speed counter-current chromatography, Journal of Chromatography A 1142 (2) : 115- 122

(2007) Garrard, IJ., Janaway, L. and Fisher, D., Minimising solvent usage in high speed, high loading, and high resolution isocratic dynamic extraction, Journal of Liquid Chromatography and Related Technologies 30 (2) : 151- 163

(2005) Garrard, I., Simple approach to the development of a CCC solvent selection protocol suitable for automation, Journal of Liquid Chromatography and Related Technologies 28 (12-13) : 1923- 1935

(2005) Fisher, D., Garrard, IJ., van den Heuvel, R., Sutherland, IA., Chou, FE. and Fahey, JW., Technology transfer and scale up of a potential cancer-preventive plant dynamic extraction of glucoraphanin, Journal of Liquid Chromatography and Related Technologies 28 (12-13) : 1913- 1922

Conference Papers

(2011) Fernando, S., Garrard, I., Allen, L. and Fisher, D., Successful monoclonal antibody purification using counter current chromatography (CCC): finding the balance between retention of biological activity and high resolution separation, Bioprocessing UK

(2011) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN., van den Heuvel, R. and Grudzien, L., Liquid-liquid chromatography: why a liquid stationary phase is so exciting, Separation Science Europe: New Horizons in Separation and Detection Techniques

(2011) Grudzien, L., Garrard, I., Fisher, D., Madeira, LD. and Ma, J., Isolation of small lectin from a hydroponic culture medium using two phase systems based on a thermo separation polymer, 16th International Conference on Biopartitioning and Purification (BPP2011)

(2011) Fernando, S., Allen, L., Fisher, D. and Garrard, I., Successful monoclonal antibody purification using counter current chromatography (CCC): finding the balance between retention of biological activity and high resolution separation, 16th International Conference on Biopartitioning and Purification (BPP2011)

(2010) Fernando, S., Garrard, I., Allen, L. and Fisher, D., Monoclonal antibody purification using robotic phase system selection coupled with counter current chromatography, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2010) Garrard, I., Fernando, S., Grudzien, L. and Fisher, D., Use of robotics for two phase solvent selection in countercurrent chromatography, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2010) Grudzien, L., Fisher, D., Garrard, I., Archer, I., Carr, R. and Fotheringham, I., Resolution and purification of a DL-amino acid by biocatalysis in countercurrent chromatography used as a reactor–separator, th International Symposium on Countercurrent Chromatography (CCC2010)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Scale up in countercurrent chromatography, CCC Workshop

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., CCC solvent systems for natural products separations, CCC Workshop

(2009) Fernando, S., Garrard, I., Allen, L. and Fisher, D., Monoclonal antibody purification using robotic phase systems election coupled with counter current chromatography, Bioprocessing UK

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., High capacity CCC for fast isolation of natural products, Royal Society of Chemistry Conference "Functional Molecules from Natural Sources"

(2009) Fernando, S., Garrard, I., Allen, L. and Fisher, D., Monoclonal antibody purification using robotic phase system selection coupled with counter current chromatography, 15th International Conference on Biopartitioning and Purification (BPP2009)

(2009) Garrard, I., Dermiki, M., Fisher, D. and Jauregi, P., Use of countercurrent chromatography and colloidal gas aphrons for the separation of two dye molecules, 15th International Conference on Biopartitioning and Purification (BPP2009)

(2009) Grudzien, L., Garrard, I., van den Heuvel, R., Archer, I., Carr, R., Fotheringham, I. and Fisher, D., Resolution and purification of a DL-amino acid by biocatalyisis in ATPS in a reactor-separator, 15th International Conference on Biopartitioning and Purification (BPP2009)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., The role of counter-current chromatography in the fractionation and identification of plant based extracts, International Exhibition-Congress on Chemical Engineering, Environmental Protection and Biotechnology (ACEMA2009)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Developing a large scale purification protocol, 4th International Conference on Bioprospecting (BIOPROSP2009)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Large scale purification of biomolecules: advantages of a liquid stationary phase, 4th International Conference on Bioprospecting (BIOPROSP2009)

(2008) Garrard, I. and Grudzien, L., Two case studies of HILIC chromatography, hydrophilic interaction chromatography, Seminar run by SeQuant and HiChrom

(2008) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Dynamic extraction: an innovative, scaleable processing technology, Scientific Update Conference, The Scale Up of Chemical Processes

(2008) Garrard, I., Sutherland, I., Fisher, D. and Ignatova, SN., The advanced bioprocessing centre: the liquid stationary phase, BioWednesday London Biotechnology Network & UK Trade and Investment

(2008) Garrard, I., Sutherland, I., Fisher D. and Ignatova, SN., Isolating natural product bioactives: recent advances, Kings Kew Square

(2008) Garrard, I., Sutherland, I., Fisher, D. and Ignatova, SN., One step natural product purifications, 6th International Symposium on Chromatography of Natural Products

Book Chapters

(2011) Garrard, IJ. and Fisher, D., High capacity countercurrent chromatography for fast isolation of natural products. In: Wrigley, SK., Thomas, R., Nicholson, N. and Bedford, C. eds. Functional molecules from natural sources. Cambridge : Royal Society of Chemistry 90- 100

(2007) Garrard, IJ., The purification of biomolecules by countercurrent chromatography. In: Subramanian, G. ed. Bioseparation and bioprocessing: A handbook, 2 volume set, 2nd, completely revised and enlarged edition. Verlag Gmbh : Wiley-VCH

Page last updated: Friday 29 August 2014