Svetlana Ignatova

Contact

Heinz Wolff 001 Brunel University Uxbridge UB8 3PH United Kingdom

Tel: +44 (0)1895 266911 Email: svetlana.ignatova@brunel.ac.uk

About Svetlana

Dr Svetlana Ignatova is a senior applications scientist at Brunel Institute for Bioengineering (BIB), Brunel University, West London, UK. She specialises in separation and concentration techniques, particularly those involving liquid-liquid extraction and chromatography. After she graduated from Chemistry Department of Moscow State University (Russia) in 1993, she started her research career by specialising in Counter-Current Chromatography (CCC), focussing on CCC theory and its application to inorganic and radioactive analysis. Pioneering studies on the hydrodynamics of two-phase liquid systems in CCC were the foundation of her PhD, awarded in 2001 from the Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences. Later this research continued within a European INTAS programme between Russia, UK (Brunel Institute for Bioengineering (BIB) at Brunel University) and France ( Université Claude Bernard). In 2003, Dr Ignatova joined Brunel University, playing an important role in BIB’s development of CCC technology from “home-made” to “industrial competitive” and in its scale-up from analytical to pilot scale through the various separation trials carried out for the National Cancer Institute (USA), Pfizer, Syngenta, Shell and GSK. Her current research interests focus on new applications of novel liquid-liquid extraction technology and in particular: herbal extract separations, enantiomers/chiral separations, ionic liquids and nanoparticles. She is also performing research on novel processing techniques which become possible when both mobile and stationary phases are liquid. One example of this is intermittent counter-current extraction (ICcE), a patented process which can concentrate and isolate active leads from natural products including Chinese herbal medicine. Dr Ignatova is currently involved in a number of collaborative exchange programmes with China the most active being with Tsinghua University ( Beijing, China) and Sichuan University (Chengdu, China). Recently Dr Ignatova has been appointed secretary of sub-group WP7 (regulatory issues) of “Good Practice in Traditional Chinese Medicine (GP-TCM) in the post genomic era” as part of EU Framework 7 on Globalization of TCM and is the senior applications scientist on a £1.5m UK government funded high value manufacturing research program on “scalable technology for the extraction of pharmaceuticals (STEP)” with a consortium including GSK, Pfizer and Dynamic Extractions.

Research Area

Bioprocess Engineering

Publications

Journal Papers

(2011) Zhang, M., Ignatova, S., Hu, P., Liang, Q., Wang, Y., Sutherland, IA., Wujun, F. and Luo, G., Cost-efficient and process-efficient separation of geniposide from Gardenia jasminoides Ellis by high-performance counter-current chromatography, Journal of Chromatography B submitted

(2011) DeAmicis, C., Edwards, NA., Giles, MB., Harris, GH., Hewitson, P., Janaway, L. and Ignatova, S., Comparison of preparative reversed phase liquid chromatography and countercurrent chromatography for the kilogram scale purification of crude spinetoram insecticide, Journal of Chromatography A 1218 (36) : 6122- 6127

(2011) Zhang, M., Ignatova, S., Hu, P., Liang, Q., Wang, Y., Luo, G., Wu Jun, F. and Sutherland, I., Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study, Journal of Chromatography A 1218 (36) : 6031- 6037

(2011) Zhang, M., Ignatova, S., Hu, P., Liang, Q., Wang, Y., Sutherland, IA., Wujun, F. and Luo, G., Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study, Journal of Chromatography A 1218 (36) : 6031- 6037

(2011) Zhang, M., Ignatova, S., Hu, P., Liang, Q., Wang, Y., Luo, G., Wu Jun, F. and Sutherland, I., Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone iia and cryptotanshinone from salvia miltiorrhiza bunge as a case study, Journal of Chromatography A 1218 (36) : 6031- 6037

(2011) Ignatova, S., Hewitson, P., Mathews, B. and Sutherland, I., Evaluation of dual flow counter-current chromatography and intermittent counter-current extraction, Journal of Chromatography A 1218 (36) : 6102- 6106

(2011) Ignatova, S., Sumner, N., Colclough, N. and Sutherland, I., Gradient elution in counter-current chromatography: A new layout for an old path, Journal of Chromatography A 1218 (36) : 6053- 6060

(2011) Hewitson, P., Ignatova, S. and Sutherland, I., Intermittent counter-current extraction-effect of the key operating parameters on selectivity and throughput, Journal of Chromatography A 1218 (36) : 6072- 6078

(2011) Sutherland, I., Ignatova, S., Hewitson, P., Janaway, L., Wood, P., Edwards, N., Harris, G., Guzlek, H., Keay, D., Freebairn, K., Johns, D., Douillet, N., Thickitt, C., Vilminot, E. and Mathews, B., Scalable technology for the extraction of pharmaceutics (STEP): The transition from academic knowhow to industrial reality, Journal of Chromatography A 1218 (36) : 6114- 6121

(2010) Ignatova, S., Hawes, D., van den Heuvel, R., Hewitson, P. and Sutherland, IA., A new non-synchronous preparative counter-current centrifuge – the next generation of dynamic extraction/chromatography devices with independent mixing and settling control, which offer a step change in efficiency, Journal of Chromatography A 1217 (1) : 34- 39

(2010) Qi, X., Ignatova, S., Luo, G., Liang, Q., Jun, FW., Wang, Y. and Sutherland, I., Preparative isolation and purification of ginsenosides Rf, Re, Rd and Rb1 from the roots of Panax ginseng with a salt/containing solvent system and flow step-gradient by high performance counter-current chromatography coupled with an evaporative light scattering detector, Journal of Chromatography A 1217 (13) : 1995- 2001

(2009) Berthod, A., Ignatova, S. and Sutherland, IA., Advantages of a small-volume counter-current chromatography column, Journal of Chromatography A 1216 (19) : 4169- 4175

(2009) Hewitson, P., Ignatova, S., Ye, H., Chen, L. and Sutherland, I., Intermittent counter-current extraction as an alternative approach to purification of Chinese herbal medicine, Journal of Chromatography A 1216 (19) : 4187- 4192

(2009) Rubio, N., Ignatova, S., Minguillón, C. and Sutherland, IA., Multiple dual mode countercurrent chromatography applied to chiral separations using a (S)-naproxen derivative as chiral selector, Journal of Chromatography A 1216 (48) : 8505- 8511

(2009) Sutherland, IA., Hewitson, P. and Ignatova, S., New 18-l process-scale counter-current chromatography centrifuge, Journal of Chromatography A 1216 (19) : 4201- 4205

(2009) Ye, H., Ignatova, S., Peng, A., Chen, L. and Sutherland, IA., Optimising resolution for a preparative separation of Chinese herbal medicine using a surrogate model sample system, Journal of Chromatography A 1216 (26) : 5101- 5105

(2009) Zhang, M., Ignatova, S., Liang, Q., Wu Jun, F., Sutherland, I., Wang, Y. and Luo, G., Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography, Journal of Chromatography A 1216 (18) : 3869- 3873

(2009) Sutherland, IA., Hewitson, P. and Ignatova, S., Scale-up of counter-current chromatography: Demonstration of predictable isocratic and quasi-continuous operating modes from the test tube to pilot/process scale, Journal of Chromatography A 1216 (50) : 8787- 8792

(2008) Ye, H., Ignatova, S., Luo, H., Li, Y., Peng, A., Chen, L. and Sutherland, IA., Preparative separation of a terpenoid and alkaloids from Tripterygium wilfordii Hook. f. using high-performance counter-current chromatography: comparison of various elution and operating strategies, Journal of Chromatography A 1213 (2) : 145- 153

(2007) Wood, P., Ignatova, S., Janaway, L., Keay, D., Hawes, D., Garrard, I. and Sutherland, IA., Counter-current chromatography separation scaled up from an analytical column to a production column, Journal of Chromatography A 1151 (1-2) : 25- 30

(2007) Ignatova, S., Wood, P., Hawes, D., Janaway, L., Keay, D. and Sutherland, I., Feasibility of scaling from pilot to process scale, Journal of Chromatography A 1151 (1-2) : 20- 24

(2007) Chen, LJ., Zhang, Q., Yang, GL., Fan, LY., Tang, J., Garrard, I., Ignatova, S., Fisher, D. and Sutherland, IA., Rapid purification and scale-up of honokiol and magnolol using high-capacity high-speed counter-current chromatography, Journal of Chromatography A 1142 (2) : 115- 122

(2005) Sutherland, I., Hawes, D., Ignatova, S., Janaway, L. and Wood, P., Review of progress toward the industrial scale-up of CCC, Journal of Liquid Chromatography and Related Technologies 28 (12-13) : 1877- 1891

(2004) Kostanian, AE., Berthod, A., Ignatova, SN., Maryutina, TA., Spivakov, BY. and Sutherland, IA., Countercurrent chromatographic separation: a hydrodynamic approach developed for extraction columns, Journal of Chromatography A 1040 (1) : 63- 72

(2004) Shpigun, OA., Pirogov, AV., Ivanov, AV., Maryutina, TA. and Ignatova, SN., HPLC systems and their use in liquid chromatography with free stationary phase, Russian Journal of Physical Chemistry A 78 (6) : 975- 979

(2003) Ignatova, SN. and Sutherland, IA., A fast, effective method of characterizing new phase systems in CCC, Journal of Liquid Chromatography and Related Technology 26 (9-10) : 1551- 1564

(2003) Janaway, L., Hawes, D., Ignatova, S., Wood, P. and Sutherland, IA., A new small coil-volume CCC instrument for direct interfacing with MS, Journal of Liquid Chromatography and Related Technologies 26 (9-10) : 1345- 1354

(2003) Maryutina, TA., Ignatova, SN. and Sutherland, IA., Organic phase retention in CCC: Effect of temperature, tubing material, tubing bore, mobile phase flow, and the addition of an extraction agent, Journal of Liquid Chromatography & Related Technology 26 (9-10) : 1537- 1550

(2003) Maryutina, TA., Ignatova, SN., Spivakov, BY. and Sutherland, IA., The efficiency of substance separation in countercurrent liquid chromatography, Journal of Analytical Chemistry 58 (8) : 762- 767 Download publication

(2003) Maryutina, TA., Ignatova, SN., Spivakov, BY. and Sutherland, IA., The efficiency of substance separation in liquid chromatography with a support-free stationary phase, Zhurnal Analiticheskoj Khimii 58 (8) : 855- 861

(2002) Maryutina, TA., Ignatova, SN. and Spivakov, BY., Extractant effect on the retention of the stationary phase in a rotating coiled column, Journal of Analytical Chemistry 57 (5) : 419- 422 Download publication

(2002) Spivakov, BY., Maryutina, TA., Fedotov, PS., Ignatova, SN., Katasonova, ON., Dahmen, J. and Wennrich, R., Separation of substances in rotating coiled columns: From trace elements to microparticles, Journal of Analytical Chemistry 57 (10) : 928- 934

(2001) Ignatova, SN. and Volynskii, AB., Determination of cadmium, cobalt, manganese, copper, nickel, and chromium in concentrated solutions of calcium chloride by electrothermal atomic absorption spectrometry, Journal of Analytical Chemistry 56 (11) : 1015- 1019

(2001) Ignatova, SN., Maryutina, TA. and Spivakov, B., Effect of physicochemical properties of two-phase liquid systems on the retention of stationary phase in a CCC column, Journal of Liquid Chromatography & Related Technologies 24 (11 & 12) : 1655- 1668

(2001) Ignatova, SN., Maryutina, TA., Spivakov, BY. and Karandashev, VK., Group separation of trace rare-earth elements by countercurrent chromatography for their determination in high-purity calcium chloride, Fresenius' Journal of Analytical Chemistry 370 (8) : 1109- 1113

(1999) Maryutina, TA., Ignatova, SN., Fedotov, PS. and Spivakov, BY., Effect of physicochemical properties of two-phase liquid systems on the retention of the organic phase in a rotating coil column, Journal of Analytical Chemistry 54 (8) : 731- 738

(1998) Maryutina, TA. and Ignatova, SN., Countercurrent chromatography for the preconcentration and separation of inorganic compounds: Influence of physicochemical properties of two-phase liquid systems on the retention of the stationary phase, Journal of Analytical Chemistry 53 (8) : 740- 745

(1998) Maryutina, TA., Ignatova, SN., Fedotov, PS., Spivakov, BY. and Thiébaut, D., Influence of composition and some physico-chemical properties of two- phase liquid systems on the stationary phase retention in a coil planet centrifuge, Journal of Liquid Chromatography and Related Technologies 21 (1-2) : 19- 37

Conference Papers

(2011) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN., van den Heuvel, R. and Grudzien, L., Liquid-liquid chromatography: why a liquid stationary phase is so exciting, Separation Science Europe: New Horizons in Separation and Detection Techniques

(2011) Ignatova, SN., Role of countercurrent chromatography in modernisation of Chinese herbal medicines, World Congress of Chinese Medicine (WCCM2011)

(2011) Sutherland, IA., Ignatova, SN., Simmonds, M. and Sendker, J., Good practice in TCMs (GPTCM): preliminary results on the quality of botanical origin, processing and extraction information contained in papers on Cornus Officinalis in the last three years, The 10th Meeting of Consortium for Globilisation of Chinese Medicine (CGCM2011)

(2011) Ignatova, SN., Douillet, N., Hewitson, P., Thickitt, C., Viliminot, E., Johns, D., Freebairn, K., Sutherland, I., Edwards, N. and Harris, G., API recovery from pharmaceutical waste streams by high performance counter-current chromatography (HPCCC) and intermittent counter-current extraction (ICcE), International Symposium, Exhibit and Workshops on Preparative and Process Chromatography (PREP 2011)

(2010) Ignatova, SN., Hewitson, P. and Sutherland, IA., New ways to solve old problems: purification of waste streams, 13th Symposium on Preparative and Industrial Chromatography and Allied Techniques (SPICA2010)

(2010) Hewitson, P., Ignatova, SN. and Sutherland, IA., Effect of the key operating parameters on the efficiency of intermittent counter-current extraction, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2010) Zhang, M., Ignatova, SN., Hu, P., Liang, Q., Wang, Y., Luo, G., Wujun, F. and Sutherland, IA., Exploiting counter-current chromatography as a green process: purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2010) Ignatova, SN., Sumner, N., Colclough, N. and Sutherland, IA., Gradient elution in counter-current chromatography: a new layout for an old path, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2010) Sutherland, IA., Ignatova, SN., Hewitson, P., Janaway, L., Wood, P., Edwards, N., Guzlek, H., Keay, D., Freebairn, K. and Johns, D., Scalable technology for the extraction of pharmaceutics (STEP): the transition from academic knowhow to industrial reality, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2010) Ignatova, SN., Hewitson, P. and Sutherland, IA., χEvaluation of dual flow counter-current chromatography and intermittent counter-current extraction, 6th International Symposium on Countercurrent Chromatography (CCC2010)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., CCC solvent systems for natural products separations, CCC Workshop

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Scale up in countercurrent chromatography, CCC Workshop

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., High capacity CCC for fast isolation of natural products, Royal Society of Chemistry Conference "Functional Molecules from Natural Sources"

(2009) Rubio, N., Ignatova, SN., Sutherland, IA. and Minguillon, C., Multiple dual-mode counter-current chromatography applied to chiral separations, 15th International Conference on Biopartitioning and Purification (BPP2009)

(2009) Hawes, D., Ignatova, SN., van den Heuvel, R. and Sutherland, IA., Non-synchronous preparative CCC – the next generation counter-current centrifuge where phase separation and mixing can be independently controlled, 15th International Conference on Biopartitioning and Purification (BPP2009)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., The role of counter-current chromatography in the fractionation and identification of plant based extracts, International Exhibition-Congress on Chemical Engineering, Environmental Protection and Biotechnology (ACEMA2009)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Developing a large scale purification protocol, 4th International Conference on Bioprospecting (BIOPROSP2009)

(2009) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Large scale purification of biomolecules: advantages of a liquid stationary phase, 4th International Conference on Bioprospecting (BIOPROSP2009)

(2008) Garrard, I., Sutherland, I., Fisher, D., Ignatova, SN. and van den Heuvel, R., Dynamic extraction: an innovative, scaleable processing technology, Scientific Update Conference, The Scale Up of Chemical Processes

(2008) Hewitson, P., Ignatova, SN., Ye, H., Chen, L. and Sutherland, IA., Intermittent counter-current extraction as an alternative approach to purification of chinese herbal medicine, 5th International Conference on Countercurrent Chromatography (CCC2008)

(2008) Hawes, D., Ignatova, SN., van den Heuvel, R. and Sutherland, IA., Non-synchronous preparative CCC - the next generation countercurrent centrifuge where phase separation and mixing can be independently controlled, 5th International Conference on Countercurrent Chromatography (CCC2008)

(2008) Ye, H., Ignatova, SN., Chen, L. and Sutherland, IA., Optimising resolution for a preparative separation of Chinese herbal medicine using a surrogate model sample system, 5th International Conference on Countercurrent Chromatography (CCC2008)

Book Chapters

(1999) Spivakov, BY., Maryutina, TA., Fedotov, PS. and Ignatova, SN., Different two-phase liquid systems for inorganic separations by countercurrent chromatography. In: Bond, AH., Dietz, ML. and Rogers, RD. eds. Metal-Ion Separation and Preconcentration: Progress and Opportunities. American Chemical Society (716) : 333- 346