Suleman, S., Fawaz, S., Roberts, T., Ellison, S., Bigger, B. and Themis, M. (2024) 'Optimised protocols to generate high titre lentiviral vectors using a novel transfection agent enabling extended HEK293T culture following transient transfection and suspension culture'. Journal of Virological Methods, 325 (April 2024). pp. 1 - 9. ISSN: 0166-0934 Open Access Link

Suleman, S., Fawaz, S., Roberts, T., Bigger, B., Ellison, S. and Themis, M. (2022) 'An optimised protocol to generate high titre lentiviral vectors by extended HEK293T culture following transient transfection and suspension culture'.ESGCT 29th Annual Congress In collaboration with BSGCT. Edinburgh, UK. 25 - 14 October. Human Gene Therapy. pp. A103 - A103. ISSN: 1043-0342 Open Access Link

Suleman, S., Payne, A., Bowden, J., Al Haque, S., Zhan, M., Fawax, S., Khalifa, MS., Jobling, S., Hay, D., Franco, M., Fronza, R., Wang, W., Strobel-Freidekind, O., Deichmann, A., Takeuchi, Y., Waddington, SN., Gil-Farina, I., Schmidt, M. and Themis, M. et al. (2022) 'HIV- 1 lentivirus tethering to the genome is associated with transcription factor binding sites found in genes that favour virus survival'. Gene Therapy (Basingstoke), 29 (12). pp. 720 - 729. ISSN: 0969-7128 Open Access Link

Suleman, S., Schrubaji, K., Filippou, C., Ignatova, S., Hewitson, P., Huddleston, J., Karda, R., Waddington, SN. and Themis, M. et al. (2021) 'Rapid and inexpensive purification of adenovirus vectors using an optimised aqueous two-phase technology'. Journal of Virological Methods, 299. pp. 1 - 9. ISSN: 0166-0934 Open Access Link

Lucendo-Villarin, B., Rashidi, H., Alhaque, S., Fischer, L., Meseguer-Ripolles, J., Wang, Y., O'farrelly, C., Themis, M. and Hay, DC. et al. (2019) 'Serum free production of three-dimensional human hepatospheres from pluripotent stem cells'. Journal of Visualized Experiments, 2019 (149). pp. 1 - 8. ISSN: 1940-087X Open Access Link

Tijani, M., Munis, AM., Perry, C., Sanber, K., Ferraresso, M., Mukhopadhyay, T., Themis, M., Nisoli, I., Mattiuzzo, G., Collins, MK. and Takeuchi, Y. et al. (2018) 'Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes'. Molecular Therapy - Methods and Clinical Development, 10. pp. 303 - 312. ISSN: 2329-0501 Open Access Link

Themis, M. (2018) 'Three-dimensional cell culture: From evolution to revolution'. Philos Trans R Soc Lond B Biol Sci, 373 (1750). pp. 20170216 - 20170216. ISSN: 0962-8436 Open Access Link

Khonsari, H., Schneider, M., Al-Mahdawi, S., Chianea, YG., Themis, M., Parris, C., Pook, M. and Themis, M. et al. (2016) 'Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts'. Nature Gene Therapy, 23 (12). pp. 846 - 856. ISSN: 0969-7128 Open Access Link

Ghorbani, M., Themis, M. and Payne, AM. (2015) 'Genome wide classification and characterisation of CpG sites in cancer and normal cells'. Computers in Biology and Medicine, 68. pp. 57 - 66. ISSN: 0010-4825 Open Access Link

Condiotti, R., Goldenberg, D., Giladi, H., Schnitzer-Perlman, T., Waddington, SN., Buckley, SMK., Heim, D., Cheung, W., Themis, M., Coutelle, C., Simerzin, A., Osejindu, E., Wege, H., Themis, M. and Galun, E. et al. (2014) 'Transduction of fetal mice with a feline lentiviral vector induces liver tumors which exhibit an E2F activation signature'. Molecular Therapy, 22 (1). pp. 59 - 68. ISSN: 1525-0016 Open Access Link

Nowrouzi, A., Arens, A., Paruzynski, A., Schmidt, M., Cheung, WT., Al-Allaf, F., Gregory, L., Dighe, N., Ruthe, A., Coutelle, C., Themis, M., Li, T., Zhang, X., Waddington, SN., Buckley, SMK., Osejindu, E., Reja, S., Roberts, TP., Newbold, RF., Von Kalle, C., Wang, Y., Themes, M., Thrasher, AJ., Holder, M., Bigger, B., Montini, E. and Andrews, R. et al. (2013) 'The fetal mouse is a sensitive genotoxicity model that exposes lentiviral-associated mutagenesis resulting in liver oncogenesis'. Molecular Therapy, 21 (2). pp. 324 - 337. ISSN: 1525-0016 Open Access Link

Themis, M. (2012) 'Monitoring for potential adverse effects of prenatal gene therapy: Genotoxicity analysis in vitro and on small animal models ex vivo and in vivo'. Methods in Molecular Biology, 891. pp. 341 - 370. ISSN: 1064-3745

Coutelle, C., Waddington, SN. and Themis, M. (2012) 'Monitoring for potential adverse effects of prenatal gene therapy: Mouse models for developmental aberrations and inadvertent germ line transmission'. Methods in Molecular Biology, 891. pp. 329 - 340. ISSN: 1064-3745

David, AL., Abi-Nader, KN., Weisz, B., Shaw, SWS., Themis, M., Cook, T., Coutelle, C., Rodeck, CH. and Peebles, DM. et al. (2010) 'ULTRASONOGRAPHIC DEVELOPMENT OF THE FETAL SHEEP STOMACH AND EVALUATION OF EARLY GESTATION ULTRASOUND-GUIDED IN UTERO I NTRAGASTRIC INJECTION'. TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY, 49 (1). pp. 23 - 29. ISSN: 1028-4559

Kennea, NL., Waddington, SN., Chan, J., O'Donoghue, K., Yeung, D., Taylor, DL., Al-Allaf, FA., Pirianov, G., Themis, M., Edwards, AD., Fisk, NM. and Mehmet, H. et al. (2009) 'Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype'. Cell Cycle, 8 (7). pp. 1069 - 1079. ISSN: 1538-4101 Open Access Link

Buckley, SMK., Waddington, SN., Jezzard, S., Bergau, A., Themis, M., MacVinish, LJ., Cuthbert, AW., Colledge, WH. and Coutelle, C. et al. (2008) 'Intra-amniotic delivery of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred CFTR-knockout mice'. Molecular Therapy, 16 (5). pp. 819 - 824. ISSN: 1525-0016 Open Access Link

David, AL., Weisz, B., Gregory, L., Themis, M., Cook, T., Roubliova, X., Deprest, J., Coutelle, C., Rodeck, CH. and Peebles, DM. et al. (2006) 'Ultrasound-guided injection and occlusion of the trachea in fetal sheep'. Ultrasound in Obstetrics and Gynecology, 28 (1). pp. 82 - 88. ISSN: 0960-7692

David, AL., Peebles, DM., Gregory, L., Waddington, SN., Themis, M., Weisz, B., Ruthe, A., Lawrence, L., Cook, T., Rodeck, CH. and Coutelle, C. et al. (2006) 'Clinically applicable procedure for gene delivery to fetal gut by ultrasound-guided gastric injection: Toward prenatal prevention of early-onset intestinal diseases'. Human Gene Therapy, 17 (7). pp. 767 - 779. ISSN: 1043-0342

David, A., Weisz, B., Ruthe, A., Peebles, DM., Coutelle, C., Rodeck, CH. and Themis, M. et al. (2006) 'Gene transfer to human fetal tissues in vitro'. BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 113 (7). pp. 863 - 863. ISSN: 1470-0328

Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, LG., Nivsarkar, M., Themis, M., Holder, MV., Buckley, SMK., Dighe, N., Ruthe, AT., Mistry, A., Bigger, B., Rahim, A., Nguyen, TH., Trono, D., Thrasher, AJ. and Coutelle, C. et al. (2006) 'Erratum: "Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice" (Molecular Therapy (2005) vol. 12 (763-771) 10.1016/j.ymthe.2005.07.358)'. Molecular Therapy, 13 (4). pp. 830. ISSN: 1525-0016

Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, LG., Nivsarkar, M., Themis, M., Holder, MV., Buckley, SMK., Dighe, N., Ruthe, AT., Mistry, A., Bigger, B., Rahim, A., Nguyen, TH., Trono, D., Thrasher, AJ. and Coutelle, C. et al. (2006) 'Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice (vol 12, pg 763, 2005)'. Molecular Therapy, 13 (4). pp. 830 - 830. ISSN: 1525-0024

Tolmachov, O., Ma, YL., Themis, M., Patel, P., Spohr, H., Macleod, KT., Ullrich, ND., Kienast, Y., Coutelle, C. and Peters, NS. et al. (2006) 'Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro'. BMC Cardiovascular Disorders, 6 (1). pp. 25 - 25. ISSN: 1471-2261 Open Access Link

Bigger, BW., Siapati, EK., Mistry, A., Waddington, SN., Nivsarkar, MS., Jacobs, L., Perrett, R., Holder, MV., Ridler, C., Kemball-Cook, G., Ali, RR., Forbes, SJ., Coutelle, C., Wright, N., Alison, M., Thrasher, AJ., Bonnet, D. and Themis, M. et al. (2006) 'Permanent partial phenotypic correction and tolerance in a mouse model of hemophilia B by stem cell gene delivery of human factor IX.'. Gene Therapy, 13 (2). pp. 117 - 126. ISSN: 0969-7128

Coutelle, C., Themis, M., Waddington, SN., Buckley, SMK., Gregory, LG., Nivsarkar, MS., David, AL., Peebles, D., Weisz, B. and Rodeck, C. et al. (2005) 'Gene therapy progress and prospects: Fetal gene therapy - First proofs of concept - Some adverse effects'. Gene Therapy, 12 (22). pp. 1601 - 1607. ISSN: 0969-7128

Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, YH., Al-Allaf, F., Gregory, L., Nivsarkar, M., Themis, M., Holder, M., Buckley, SMK., Dighe, N., Ruthe, A., Mistry, A., Bigger, B., Thrasher, A. and Coutelle, C. et al. (2005) 'Oncogenesis following delivery of a non-primate lentiviral gene therapy vector to fetal mice'.Autumn Meeting of the British-Toxicology-Society. Univ Bath, Bath, ENGLAND. 19 - 20 September. ELSEVIER IRELAND LTD. pp. 233 - 233. ISSN: 0300-483X

Siapati, EK., Bigger, BW., Miskin, J., Chipchase, D., Parsley, KL., Mitrophanous, K., Themis, M., Thrasher, AJ. and Bonnet, D. et al. (2005) 'Comparison of HIV- and EIAV-based vectors on their efficiency in transducing murine and human hematopoietic repopulating cells'. Molecular Therapy, 12 (3). pp. 537 - 546. ISSN: 1525-0016

Weisz, B., David, AL., Gregory, LG., Perocheau, D., Ruthe, A., Waddington, SN., Themis, M., Cook, T., Coutelle, C., Rodeck, CH. and Peebles, DM. et al. (2005) 'Targeting the respiratory muscles of fetal sheep for prenatal gene therapy for Duchenne muscular dystrophy'. American Journal of Obstetrics and Gynecology, 193 (3). pp. 1105 - 1109. ISSN: 1097-6868

Weisz, B., David, AL., Gregory, LG., Perocheau, D., Ruthe, A., Waddington, SN., Themis, M., Cook, T., Coutelle, C., Rodeck, CH. and Peebles, DM. et al. (2005) 'Targeting the respiratory muscles of fetal sheep for prenatal gene therapy for Duchenne muscular dystrophy'. American Journal of Obstetrics and Gynecology, 193 (3 SUPPL.). pp. 1105 - 1109. ISSN: 0002-9378

Buckley, SM., Waddington, SN., Jezzard, S., Lawrence, L., Schneider, H., Holder, MV., Themis, M. and Coutelle, C. et al. (2005) 'Factors influencing adenovirus-mediated airway transduction in fetal mice'. Molecular Therapy, 12 (3). pp. 484 - 492. ISSN: 1525-0016

Al-Allaf, FA., Tolmachov, O., Themis, M. and Coutelle, C. (2005) 'Coupled analysis of bacterial transformants and ligation mixture by duplex PCR enables detection of fatal instability of a nascent recombinant plasmid'. Journal of Biochemical and Biophysical Methods, 64 (2). pp. 142 - 146. ISSN: 0165-022X

Bigger, BW., Waddington, SN., Jones, N., Buch, M., Jacobs, L., Perrett, R., Nivsarkar, M., Watt, SM., Wood, K. and Themis, M. et al. (2005) 'Non-myeloablative stem cell gene delivery effectively establishes long-term gene expression and mixed chimerism in immunocompetent mice'.34th Annual Meeting of the International-Society-for-Experimental-Hematology. Univ Glasgow, Glasgow, SCOTLAND. 30 - 2 August. ELSEVIER SCIENCE INC. pp. 41 - 42. ISSN: 0301-472X

Waddington, SN., Kramer, MG., Hernandez-Alcoceba, R., Buckley, SM., Themis, M., Coutelle, C. and Prieto, J. et al. (2005) 'In utero gene therapy: Current challenges and perspectives'. Molecular Therapy, 11 (5). pp. 661 - 676. ISSN: 1525-0016

Al-Allaf, F., Tolmachov, O., Themis, M. and Coutelle, C. (2005) 'Accurate size gauging of ExoIII/S1-generated deletions by PCR analysis of ligation mixtures'. Anal Biochem, 339 (2). pp. 348 - 350. ISSN: 0003-2697

Themis, M., Bennett, PR., Mehmet, H., Waddington, SN. and Buckley, SM. (2005) 'The development of a mouse model to study the effect of uterine infection on preterm labour and fetal outcome.'.52nd Annual Meeting of the Society-for-Gynecologic-Investigation. Los Angeles, CA. 23 - 26 March. ELSEVIER SCIENCE INC. pp. 259A - 259A. ISSN: 1071-5576

Weisz, B., David, A., Peebles, D., Gregory, L., Perocheau, D., Waddington, S., Themis, M., Cook, T., Coutelle, C. and Rodeck, C. et al. (2005) 'Targeting the fetal respiratory muscles for prenatal gene therapy for Duchenne muscular dystrophy'.25th Annual Meeting of the Society-for-Maternal-Fetal-Medicine. Reno, NV. 7 - 12 February. MOSBY-ELSEVIER. pp. S22 - S22. ISSN: 0002-9378

David, A., Weisz, B., Peebles, D., Roubliova, X., Perocheau, D., Themis, M., Cook, T., Coutelle, C., Deprest, J. and Rodeck, C. et al. (2005) 'Ultrasound guided placement of an occlusive balloon in the trachea of fetal sheep'.25th Annual Meeting of the Society-for-Maternal-Fetal-Medicine. Reno, NV. 7 - 12 February. MOSBY-ELSEVIER. pp. S166 - S166. ISSN: 0002-9378

Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, LG., Nivsarkar, M., Themis, M., Holder, MV., Buckley, SM., Dighe, N., Ruthe, AT., Mistry, A., Bigger, B., Rahim, A., Nguyen, TH., Trono, D., Thrasher, AJ. and Coutelle, C. et al. (2005) 'Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice.'. Molecular Therapy, 12 (4). pp. 763 - 771. ISSN: 1525-0016

Waddington, SN., Nivsarkar, MS., Mistry, AR., Buckley, SMK., Kemball-Cook, G., Mosley, KL., Mitrophanous, K., Radcliffe, P., Holder, MV., Brittan, M., Georgiadis, A., Al-Allaf, F., Bigger, BW., Gregory, LG., Cook, HT., Ali, RR., Thrasher, A., Tuddenham, EGD., Themis, M. and Coutelle, C. et al. (2004) 'Permanent phenotypic correction of hemophilia B in immunocompetent mice by prenatal gene therapy'. BLOOD, 104 (9). pp. 2714 - 2721. ISSN: 0006-4971

Gregory, LG., Waddington, SN., Holder, MV., Mitrophanous, KA., Buckley, SM., Mosley, KL., Bigger, BW., Ellard, FM., Walmsley, LE., Lawrence, L., Al-Allaf, F., Kingsman, S., Coutelle, C. and Themis, M. et al. (2004) 'Highly efficient EIAV-mediated in utero gene transfer and expression in the major muscle groups affected by Duchenne muscular dystrophy'. Gene Therapy, 11 (14). pp. 1117 - 1125. ISSN: 0969-7128

Themis, M., Gregory, LG., Waddington, SN., Holder, MV., Mitrophanous, KA., Buckley, SMK., Bigger, BW., Ellard, FE., Walmsley, LE., Radcliff, P., Mazarakis, N., Azzouz, M., Lawrence, L., Cook, T., Allaf, FA., Kingsman, S. and Coutelle, C. et al. (2004) 'The fetal approach: A novel therapy for the treatment of musculo-skeletal disease'.7th Annual Meeting of the American-Society-of-Gene-Therapy. Minneapolis, MN. 2 - 6 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S91 - S91. ISSN: 1525-0016

Bigger, BW., Siapati, EK., Mistry, A., Waddington, SN., Nivsarkar, MS., Holder, MV., Ridler, C., Kemball-Cook, G., Ali, RR., Forbes, S., Coutelle, C., Wright, N., Alison, M., Thrasher, A., Bonnet, D. and Themis, M. et al. (2004) 'Induction of immune tolerance and phenotypic rescue of hemophilic mice by stem cell mediated gene transfer'.7th Annual Meeting of the American-Society-of-Gene-Therapy. Minneapolis, MN. 2 - 6 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S64 - S64. ISSN: 1525-0016

Ma, YI., Tolmachov, O., Themis, M., Spohr, H., Kienast, Y., Coutelle, C. and Peters, NS. et al. (2004) 'Field action potential activation and propagation in cocultured cardiac myocytes and Cx43 transduced skeletal myoblasts in a multi-electrode array'.Annual Conference of the British-Cardiac-Society. Manchester, ENGLAND. 24 - 27 May. B M J PUBLISHING GROUP. pp. A37 - A37. ISSN: 1355-6037

Waddington, SN., Buckley, SM., Bernloehr, C., Bossow, S., Ungerechts, G., Cook, T., Gregory, L., Rahim, A., Themis, M., Neubert, WJ., Coutelle, C., Lauer, UM. and Bitzer, M. et al. (2004) 'Reduced toxicity of F-deficient Sendai virus vector in the mouse fetus'. Gene Ther, 11 (7). pp. 599 - 608. ISSN: 0969-7128

Bergau, A., Gregory, L., Waddington, S., Harbottle, R., Lawrence, L., Coutelle, C. and Themis, M. et al. (2004) 'Comparative analysis of the human cytokeratin K18 promoter with the CMV promoter by hydrodynamic injection of adult MF-1 mice'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S5 - S6. ISSN: 1099-498X

Bigger, BW., Siapati, KE., Mistry, A., Waddington, SN., Nivsarkar, MS., Holder, MV., Ridler, C., Kemball-Cook, G., Ali, RR., Forbes, S., Coutelle, C., Alison, M., Wright, N., Thrasher, AJ., Bonnet, D. and Themis, M. et al. (2004) 'Induction of immune tolerance and partial phenotypic correction of haermophiliac mice by lentiviral gene transfer of human factor IX to haematopoietic stem cells'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S15 - S15. ISSN: 1099-498X

Holder, MV., Waddington, SN., Gregory, LG., Kramer, MG., Prieto, J., Mitrophanous, K., Kingsman, S., Coutelle, C., Themis, M. and Bigger, BW. et al. (2004) 'Comparison of gene expression from liver specific promoter/enhancer combinations in the context of a lentiviral backbone'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S9 - S9. ISSN: 1099-498X

Gregory, LG., Waddington, SN., Holder, M., Mitrophanous, KA., Buckley, SMK., Ellard, FM., Walmsley, LE., Wells, D., Lui, K., Kingsman, S., Coutelle, C. and Themis, M. et al. (2004) 'Pseudotyped lentiviral vectors: Toward an applicability for in utero treatment of Duchenne Muscular Dystrophy'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S18 - S18. ISSN: 1099-498X

Chan, J., O'Donoghue, K., Kennea, N., de la Fuente, J., Waddington, S., Themis, M., Mehmet, H., Watt, D., Morgan, J. and Fisk, N. et al. (2004) 'Human fetal mesenchymal stem cells for intrauterine cellular therapy'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S17 - S17. ISSN: 1099-498X

Themis, M. (2004) 'An in vitro hprt locus insertion mutagenesis assay'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S37 - S37. ISSN: 1099-498X

Waddington, SN., Nivsarkar, M., Mistry, A., Buckley, SMK., Kemball-Cook, G., Mosley, KL., Mitrophanous, K., Radcliffe, P., Holder, M., Brittan, M., Georgiadis, T., Al-Allaf, F., Bigger, B., Gregory, L., Cook, HT., Ali, RR., Thrasher, A., Tuddenham, EGD., Themis, M. and Coutelle, C. et al. (2004) 'Permanent phenotypic correction of Haemophilia B in immunocompetent mice by prenatal gene therapy'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. WILEY-BLACKWELL. pp. S17 - S17. ISSN: 1099-498X