Uday Kishore

Summary

uday-kishore

I studied biology in the school, did B.Sc. in Zoology and M.Sc. in Cell Biology, and went on to carry out PhD research in molecular biology from Delhi. After a short stint at Salk Institute, San Diego as a NASA fellow, I joined in 1996 Department of Biochemistry, Oxford, first as a Wellcome Trust Overseas Fellow, and then as a Research Associate within Medical Research Council Immunochemistry Unit. At Oxford, I became interested in pattern recognizing innate immune molecules such as complement protein C1q and pulmonary surfactant proteins SP-A and SP-D. The identification of a major role of SP-D in controlling pulmonary inflammation and infection by our group took me further into host-pathogen interaction. I became involved in a range of projects that culminated in demonstrating that recombinant SP-D can have therapeutic potential again pulmonary hypersensitivity and invasive infection. Since then, I have been concentrating on clinical aspects of the above mentioned proteins that have ramifications as far as asthma, infection, autoimmunity, transplantation and a number of neonatal conditions.

My current interest lies in role of innate immunity in host defence using a model fungal pathogen, Aspergillus fumigatus. This pathogen causes allergic bronchopulmonary aspergillosis (ABPA) in immunocompetent individuals, while being capable of causing fatal invasive aspegillosis in immunocompromised subjects. Human SP-D is a pulmonary surfactant-associated innate immune molecule. Our studies have established its potent anti-microbial and anti-inflammatory properties in vitro, in vivo and ex vivo. Using a murine model of severe lung infection (invasive pulmonary aspergillosis, IPA) caused by a fungal pathogen, Aspergillus fumigatus (Afu), we found that treatment with rhSP-D can rescue mice from certain death. All untreated IPA mice died within 5-7 days, the rhSP-D treated group showed >90% survival (without any side effects). The SP-D mediated protective mechanisms include agglutination of pathogen, fungistasis, enhanced killing by phagocytes and predominant Th1 response. Increased susceptibility of SP-D gene deficient mice to IPA supports our view. Patients, who are immunocompromised or immunodeficient due to chemotherapy, cancer, transplantation or AIDS are high risk IPA groups.

Using animal models of asthma caused by Afu and dust mite, we have shown that rhSP-D has a long term therapeutic value in dampening asthmatic symptoms in mice. The therapeutic mechanisms include inhibition of allergen-IgE binding and histamine release by sensitized mast cells, dramatic downregulation of specific IgG and IgE antibodies and pulmonary and peripheral eosinophilia, a shift from pathogenic Th2 to a protective Th1 cytokine response, inhibition of mechanisms that cause airway remodelling, and apoptosis induction in sensitised eosinophils. This is consistent with inherent hypereosinophilia and Th2 predominance, and hence increased susceptibility of SP-D gene deficient mice, to allergic hypersensitivity. Thus, another important milestone in sight is to evaluate therapeutuc efficacies of a recombinant form of human SP-D in patients suffering from asthma and lung infection. I aim to further understand how SP-D modulates immune responses, and how it maintains persistence of its therapeautic effect. Using animal models of ABPA and IPA in wild type and SP-D knock out mice, we will try to understand how SP-D modulates eosinophils, dendritic cells (DC), and helper T cell polarisation to protective Th1 response that are so central to therapeutic mechanisms.

In addition, I remain interested in structural and functional aspects of the globular head region of human C1q, the first subcomponent of the classical complement pathway. The potential application of the recombinant forms of C1q globular head domains in dampening complement activation in a range of inflammatory conditions including neurodegenerative diseseaes is also being explored on my laboratory. A number of above mentioned studies have led to setting up of clinical trials, which remain at the core of my translational research.

Qualifications:

  • PhD (1995): Department of Zoology, University of Delhi and CSIR Institute of Genomics and Integrative Biology, Delhi, Indi
    Thesis: Chemical gene synthesis and expression of Human Epidermal Growth Factor in Escherichia coli.

Responsibilities

Administration Responsibilities:

Module co-ordinator:

BB2405 Genetic Engineering and Immunobiology;

BB3216 Microbial Pathogenesis

Deputy Postgraduate Admission Tutor, Biosciences

Other Responsibilities:

Personal tutor to a number of undergraduate and postgraduate students

Research area(s)

  1. Mechanisms underlying therapeutic effects of exogenous recombinant surfactant protein D (SP-D) in disease models of pulmonary hypersensitivity, inflammation and infection
  2. Host pathogen interaction studies using models such as Aspergillus fumigates, Mycobacterium tuberculosis, malaria parasite and Schistosomes.
  3. In vivo modulation of dendritic cell functions and mechanism of helper T cell polarisation by SP-D
  4. Mechanisms governing SP-D mediated survival and induction of apoptosis of eosinophils and macrophages
  5. Instructive role of collectins and complement proteins in dendritic cell-mediated handling of apoptotic cells
  6. Structure-function studies on complement protein C1q and novel members of C1q/TNF super family
  7. Generation of recombinant inhibitors of classical complement pathway
  8. Role of complement in Alzheimer’s disease and familial dementia
  9. Immunological nature of fetal-maternal interaction; involvement of surfactant proteins in parturition, pre-term birth, preeclampsia, miscarriage and intrauterine growth retardation
  10. Innate immune aspects of cancer
  11. Identification, cloning, characterisation and tolerisation of pathogenic T cells in autoimmunity using MHC class II (HLA-DR) multimer technology. 

We have recently established a protective role of SP-D in asthma and lung infection. Using an opportunistic fungal pathogen, Aspergillus fumigatus, we have also shown that SP-D treatment can be very effective in curing allergic as well as invasive (infective) aspergillosis. It is becoming increasingly clear that A. fumigatus has become a serious health concern especially in the immunocompromised or immunosuppressed individuals and there is a requirement for novel therapies against fungal infection in view of toxicity associated with current drugs and emerging cases of drug resistant strains. In this context, lung surfactant protein D (SP-D) based therapy has an excellent potential for research and development. Our research has opened avenues for further assessment of the therapeutic use of recombinant SP-D in pulmonary hypersensitivity, inflammation and infection. We are in the process of initiating Phase I clinical trial. 

Our laboratory research relies on a range of techniques and strategies including gene synthesis, cDNA cloning, recombinant protein expression in E. coli, Pichia pastoris, baculovirus, Drosophila S2 cells, mammalian cell line (HEK, CHO and COS), protein purification, site-directed mutagenesis, structure-function studies, DNA-protein and protein-protein interactions, murine models of lung allergy, inflammation, oedema and infection, immunoassays, FACS, in vitro and ex vivo cell culture, proteomics, microarray, and various microscopic techniques. 

We also have a number of collaborations in the field of protein structure (crystallography and NMR), in vivo inhibition of complement pathways, role of classical complement pathway in lupus nephritis, involvement of classical and lectin complement pathways during kidney ischemia reperfusion, induction of T cell tolerance by soluble HLA-DR, mechanism of growth inhibition by SP-D using Listeria monocytogens and Aspergillus fumigatus as microbial targets, and fetal-maternal immunity.

Membership and affiliation

Additional Publications

Books and special editions edited:

Target Pattern Recognition in Innate Immunity. 2009. Series: Advances in Experimental Medicine and Biology, Vol. 653: Landes Bioscience and Springer. ISBN: 978-1-4419-0900-8

Currently writing

Text book on Host-Pathogen Interaction (Wiley-Blackwell)

Currently editing:

1. Special Issue of Frontiers in Biosciences titled Pulmonary surfactant in human health and disease

2. Special Topic Edition on C1q family for Frontiers in Immunology

3. Microbial Pathogenesis: Infection and Immunity (Springer)

International Complement Society

European Complement Society

Complement UK

Association of Clinical Biochemists

Professional Activities or Recognition:

Pre-docstoral positions:

  • 1995: NASA Fellow at the Salk Institute for Biological Studies, La Jolla, California, U.S.A.
  • Nov, 1994-April, 1995: Visiting Student at the MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, UK.
  • 1990-94: Research Fellow at the CSIR Institute of Genomics and Integrative Biology, Delhi and Department of Zoology, University of Delhi.
  • 1986-88: Research Assistant at the CSIR Institute of Genomics and Integrative Biology, Delhi. 

Post-dosctoral positions:

  • Since May 2006: University Lecturer in Immunology at the Division of Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge, UK.
  • 2004-2005: Alexander von Humboldt International fellow at the Institute of Medical Microbiology, Justus-Leibig-University, Giessen, Germany.
  • 1999-2003: European Commission Research Scientist at the Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.
  • 1997-98: Research Associate at the MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, UK.
  • 1996: Wellcome Trust International Fellow at the Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, UK.

Awards/distinctions:

Alexander von Humboldt International Fellow, 2005

European Commission Young Scientist Award, 2002

  • MRC Investigator Award, 1998

Wellcome International Fellow, 1995

  • NASA Fellow, 1995

Professional activities/recognition (e.g. consultancy, external examiner duties)

Executive Director of Future Genetics Limited

Managing Editor of Frontiers in Biosciences

Associate Editor of Frontiers in Immunology

Editorial board: Immunology

Regular reviewer of grant applications for the Wellcome Trust, Medical Research Council UK, Research funding agencies of Singapore, Germany, India, and others.

Teaching/Supervision

Teaching

Teaching Responsibilities:

BB2405 Immunobiology

BB2216 Medical Microbiology

BB3216 Microbial Pathogenesis

BB3320 Medical Immunology

Research supervision

The Kishore Group:

Annapurna Nayak: Role of C1q in neuroinflammation and neurodegeneration associated with Alzheimer's disease and familial dementia

Asif Qaseem: Understanding immunological mechanisms involved in the control of asthma by surfactant protein SP-D

Najmunisa Karbani: Examining roles of SP-A and SP-D in foetal-maternal interaction

Lina Pednekar: Involvement of C1q and C1q receptors in drug-induced susceptibility of tumours to complement attack

Priyaa Madhukaran: Interaction of SP-A and SP-D with decidual macrophages and natural killer cells during pregnancy

Eswari Marri: Interaction of SP-A and SP-D with immune cells involved in allergic hypersensitivity

Agnes Boateng: Innate immunity and cancer

Dr Abhishek Shastri: Immunological basis of neuroinflammation and neurodegeneration

Aline Lokoto: Host-pathogen interaction in Schistosomiasis

Selected Publications

Journal articles

Ferluga, J. , Kouser, L. , Murugaiah, V. , Sim, RB.  and Kishore, U.   (Accepted)   'Potential Influences of Complement Factor H in autoimmune inflammatory and thrombotic disorders'. Molecular ImmunologyDownload publication 

Rokade, S. , Kishore, U.  and Madan, T.   (Accepted)   'Surfactant Protein D regulates murine testicular immune milieu and sperm functions'. American Journal of Reproductive ImmunologyDownload publication 

Radanova, M. , Vasilev, V. , Deliyska, B. , Kishore, U. , et al.   (2011)   'Anti-C1q autoantibodies specific against the globular domain of the C1qB-chain from patient with lupus nephritis inhibit C1q binding to IgG and CRP'. Immunobiology. doi: 10.1016/j.imbio.2011.11.007 

Kishore, U.  and Sim, RB.   (2012)   'Factor H as a regulator of the classical pathway activation'. Immunobiology, 217 (2).  pp. 162 - 168. doi: 10.1016/j.imbio.2011.07.024 

Gupta, A. , Kaul, A. , Tsolaki, AG. , Kishore, U.  and Bhakta, S.   (2012)   'Mycobacterium tuberculosis: Immune evasion, latency and reactivation'. Immunobiology, 217 (3).  pp. 363 - 374. doi: 10.1016/j.imbio.2011.07.008 

Kang, YH. , Urban, BC. , Sim, RB.  and Kishore, U.   (2012)   'Human complement Factor H modulates C1q-mediated phagocytosis of apoptotic cells.'. Immunobiology, 217 (4).  pp. 455 - 464. doi: 10.1016/j.imbio.2011.10.008 

Sudhakaran, C. , Kishore, U. , Anjana, RM. , Unnikrishnan, R.  and Mohan, V.   (2011)   'Effectiveness of sitagliptin in asian Indian patients with type 2 diabetes-an Indian tertiary diabetes care center experience'. Diabetes Technology & Therapeutics, 13 (1).  pp. 27 - 32. doi: 10.1089/dia.2010.0120 

Sumner, R. , Parton, A. , Nowicky, AV. , Kishore, U.  and Gidron, Y.   (2011)   'Hemispheric lateralisation and immune function: A systematic review of human research'. Journal of Neuroimmunology, 240-241 pp. 1 - 12. doi: 10.1016/j.jneuroim.2011.08.017 Download publication 

Nayak, A. , Pedenekar, L. , Reid, KB.  and Kishore, U.   (2011)   'Complement and non-complement activating functions of C1q: A prototypical innate immune molecule'. Innate Immunity. doi: 10.1177/1753425910396252 

Nayak, A. , Ansar, R. , Verma, SK. , Bonifati, DM.  and Kishore, U.   (2011)   'Huntington's disease: An immune perspective'. Neurology Research International, 2011 pp. 563784 - 563784. doi: 10.1155/2011/563784 Download publication 

Tan, LA. , Yang, AC. , Kishore, U.  and Sim, RB.   (2011)   'Interactions of complement proteins C1q and factor H with lipid A and Escherichia coli: Further evidence that factor H regulates the classical complement pathway'. Protein Cell, 2 (4).  pp. 320 - 332. doi: 10.1007/s13238-011-1029-y 

Verma, SK. , Ganesan, TS. , Kishore, U.  and Parker, PJ.   (2011)   'The tumor suppressor RASSF1A is a novel effector of small G protein Rap1A'. Protein Cell, 2 (3).  pp. 237 - 249. doi: 10.1007/s13238-011-1028-z 

Tan, LA. , Yu, B. , Sim, FC. , Kishore, U.  and Sim, RB.   (2010)   'Complement activation by phospholipids: The interplay of factor H and C1q'. Protein & Cell, 1 (11).  pp. 1033 - 1049. doi: 10.1007/s13238-010-0125-8 

Al-Madani, WA. , Siddiqi, NJ. , Alhomida, AS. , Khan, HA. , et al.   (2010)   'Increased urinary excretion of carnitine and acylcarnitine by mercuric chloride is reversed by 2,3-dimercapto-1-propanesulfonic acid in rats'. International Journal of Toxicology, 29 (3).  pp. 313 - 317. doi: 10.1177/1091581810364852 

Madan, T. , Reid, KB. , Clark, H. , Singh, M. , et al.   (2010)   'Susceptibility of mice genetically deficient in SP-A or SP-D gene to invasive pulmonary aspergillosis'. Molecular Immunology, 47 (10).  pp. 1923 - 1930. doi: 10.1016/j.molimm.2010.02.027 

Nayak, A. , Ferluga, J. , Tsolaki, AG.  and Kishore, U.   (2010)   'The non-classical functions of the classical complement pathway recognition subcomponent C1q'. Immunology Letters, 131 (2).  pp. 139 - 150. doi: 10.1016/j.imlet.2010.03.012 

Waters, P. , Vaid, M. , Kishore, U.  and Madan, T.   (2009)   'Lung surfactant proteins A and D as pattern recognition proteins'. Advances in Experimental Medicine and Biology, 653 pp. 74 - 97. doi: 10.1007/978-1-4419-0901-5_6 

Shrive, AK. , Martin, C. , Burns, I. , Paterson, JM. , et al.   (2009)   'Structural characterisation of ligand-binding determinants in human lung surfactant protein D: Influence of Asp325'. Journal of Molecular Biology, 394 (4-10).  pp. 776 - 788. doi: 10.1016/j.jmb.2009.09.057 Download publication 

Singh, M. , Madan, T. , Waters, P. , Sonar, S. , et al.   (2009)   'Therapeutic effects of recombinant forms of full-length and truncated human surfactant protein D in a murine model of invasive pulmonary aspergillosis'. Molecular Immunology, 46 (11-12).  pp. 2363 - 2369. doi: 10.1016/j.molimm.2009.03.019 

Kishore, U.   (2009)   'Target pattern recognition in innate immunity'. Advances in Experimental Medicine and Biology, 653 pp. ix - x. doi: 10.1007/978-1-4419-0901-5 

Roumenina, LT. , Popov, KT. , Bureeva, SV. , Kojouharova, M. , et al.   (2008)   'Interaction of the globular domain of human C1q with Salmonella typhimurium lipopolysaccharide'. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 1784 (9).  pp. 1271 - 1276. doi: 10.1016/j.bbapap.2008.04.029 Download publication 

Gadjeva, MG. , Rouseva, MM. , Zlatarova, AS. , Reid, KBM. , et al.   (2008)   'Interaction of human C1q with IgG and IgM: Revisited'. Biochemistry, 47 (49).  pp. 13093 - 13102. doi: 10.1021/bi801131h 

Mahajan, L. , Madan, T. , Kamal, N. , Singh, VK. , et al.   (2008)   'Recombinant surfactant protein-D selectively increases apoptosis in eosinophils of allergic asthmatics and enhances uptake of apoptotic eosinophils by macrophages'. International Immunology, 20 (8).  pp. 993 - 1007. doi: 10.1093/intimm/dxn058 

Ghai, R. , Waters, P. , Roumenina, LT. , Gadjeva, M. , et al.   (2007)   'C1q and its growing family'. Immunobiology, 212 (4-5).  pp. 253 - 266. doi: 10.1016/j.imbio.2006.11.001 

Tsacheva, I. , Radanova, M. , Todorova, N. , Argirova, T.  and Kishore, U.   (2007)   'Detection of autoantibodies against the globular domain of human C1q in the sera of systemic lupus erythematosus patients'. MOLECULAR IMMUNOLOGY, 44 (8).  pp. 2147 - 2151. doi: 10.1016/j.molimm.2006.09.009 

Roumenina, L. , Bureeva, S. , Kantardjiev, A. , Karlinsky, D. , et al.   (2007)   'Complement C1q-target proteins recognition is inhibited by electric moment effectors'. Journal of Molecular Recognition, 20 (5).  pp. 405 - 415. doi: 10.1002/jmr.853 

Cordery, D. , Kishore, U. , Kyes, S. , Shafi, MJ. , et al.   (2007)   'Characterisation of Plasmodium falciparum macrophage migration inhibitory factor homologue'. Journal of Infectious Diseases, 195 (6).  pp. 905 - 912. doi: 10.1086/511309 Download publication 

Sim, RB. , Kishore, U. , Villiers, CL. , Marche, PN.  and Mitchell, DA.   (2007)   'C1q binding and complement activation by prions and amyloids'. Immunobiology, 212 (4-5).  pp. 355 - 362. doi: 10.1016/j.imbio.2007.04.001 

Bonifati, DM.  and Kishore, U.   (2007)   'Role of complement in neurodegeneration and neuroinflammation'. Molecular Immunology, 44 (5).  pp. 999 - 1010. doi: 10.1016/j.molimm.2006.03.007 

Zlatarova, AS. , Rouseva, M. , Roumenina, LT. , Gadjeva, M. , et al.   (2006)   'Existence of different but overlapping IgG- and IgM-binding sites on the globular domain of human C1q'. Biochemistry, 45 (33).  pp. 9979 - 9988. doi: 10.1021/bi060539v 

Roumenina, LT. , Ruseva, MM. , Zlatarova, A. , Ghai, R. , et al.   (2006)   'Interaction of C1q with IgG1, C-reactive protein and pentraxin 3: Mutational studies using recombinant globular head modules of human C1q A, B, and C chains'. Biochemistry, 45 (13).  pp. 4093 - 4104. doi: 10.1021/bi052646f 

Kishore, U. , Greenhough, TJ. , Waters, P. , Shrive, AK. , et al.   (2006)   'Surfactant proteins SP-A and SP-D: Structure, function and receptors'. Molecular Immunology, 43 (9).  pp. 1293 - 1315. doi: 10.1016/j.molimm.2005.08.004 

Roumenina, L. , Zlatarova, A. , Olova, N. , Radanova, M. , et al.   (2005)   'Contribution of the c1q polypeptide chains in the recognition of crp'. Biotechnology and Biotechnological Equipment, 19 (2).  pp. 122 - 125. doi: 10.1080/13102818.2005.10817202 

Singh, U. , Nicholson, G. , Urban, BC. , Sargent, IL. , et al.   (2005)   'Immunological properties of human decidual macrophages - A possible role in intrauterine immunity'. Reproduction, 129 (5).  pp. 631 - 637. doi: 10.1530/rep.1.00331 

Spreadbury, I. , Kishore, U. , Beeson, D.  and Vincent, A.   (2005)   'Inhibition of acetylcholine receptor function by seronegative myasthenia gravis non-IgG factor correlates with desensitisation'. Journal of Neuroimmunology, 162 (1-2).  pp. 149 - 156. doi: 10.1016/j.jneuroim.2005.01.009 

Groeneveld, TWL. , Oroszlan, M. , Owens, RT. , Faber-Krol, MC. , et al.   (2005)   'Interactions of the extracellular matrix proteoglycans decorin and biglycan with C1q and collectins'. The Journal of Immunology, 175 (7).  pp. 4715 - 4723. 

Kishore, U. , Ghai, R. , Greenhough, TJ. , Shrive, AK. , et al.   (2005)   'Erratum to “Structural and functional anatomy of the globular domain of complement protein C1q” [Immunol. Lett. 95 (2) (2004) 113–128]'. Immunology Letters, 101 (1).  pp. 116 - 116. doi: 10.1016/j.imlet.2005.08.003 

Kishore, U. , Bernal, AL. , Kamran, MF. , Saxena, S. , et al.   (2005)   'Surfactant proteins SP-A and SP-D in human health and disease'. Arch Immunol Ther Exp (Warsz), 53 (5).  pp. 399 - 417. 

Madan, T. , Kaur, S. , Saxena, S. , Singh, M. , et al.   (2005)   'Role of collectins in innate immunity against aspergillosis'. Med Mycol, 43 Suppl 1 pp. S155 - S163. 

Madan, T. , Reid, KB. , Singh, M. , Sarma, PU.  and Kishore, U.   (2005)   'Susceptibility of mice genetically deficient in the surfactant protein (SP)-A or SP-D gene to pulmonary hypersensitivity induced by antigens and allergens of Aspergillus fumigatus'. Journal of Immunology, 174 (11).  pp. 6943 - 6954. 

Roumenina, LT. , Kantardjiev, AA. , Atanasov, BP. , Waters, P. , et al.   (2005)   'Role of Ca2+ in the electrostatic stability and the functional activity of the globular domain of human C1q'. Biochemistry, 44 (43).  pp. 14097 - 14109. doi: 10.1021/bi051186n Download publication 

Kishore, U. , Gaboriaud, C. , Waters, P. , Shrive, AK. , et al.   (2004)   'C1q and tumor necrosis factor superfamily: Modularity and versatility'. Trends in Immunology, 25 (10).  pp. 551 - 561. doi: 10.1016/j.it.2004.08.006 

Groeneveld, TWL. , Seelen, MA. , Duijs, JMGM. , Bajema, IM. , et al.   (2004)   'Anti-C1q autoantibodies deposit in glomeruli but are only pathogenic in combination with glomerular C1q-containing immune complexes'. Journal of Clinical Investigation, 114 (5).  pp. 679 - 688. doi: 10.1172/JCI200421075 

McConville, J. , Farrugia, ME. , Beeson, D. , Kishore, U. , et al.   (2004)   'Detection and characterization of MuSK antibodies in seronegative myasthenia gravis'. Annals of Neurology, 55 (4).  pp. 580 - 584. doi: 10.1002/ana.20061 

Kojouharova, MS. , Gadjeva, MG. , Tsacheva, IG. , Zlatarova, A. , et al.   (2004)   'Mutational analyses of the recombinant globular regions of human C1q A, B, and C chains suggest an essential role for arginine and histidine residues in the C1q-IgG interaction'. The Journal of Immunology, 172 (7).  pp. 4351 - 4358. 

Kishore, U. , Ghai, R. , Greenhough, TJ. , Shrive, AK. , et al.   (2004)   'Structural and functional anatomy of the globular domain of complement protein C1q'. Immunology Letters, 95 (2).  pp. 113 - 128. doi: 10.1016/j.imlet.2004.06.015 

Nauta, AJ. , Bottazzi, B. , Mantovani, A. , Salvatori, G. , et al.   (2003)   'Biochemical and functional characterization of the interaction between pentraxin 3 and C1q'. European Journal of Immunology, 33 (2).  pp. 465 - 473. doi: 10.1002/immu.200310022 

Kojouharova, MS. , Tsacheva, IG. , Tchorbadjieva, MI. , Reid, KBM.  and Kishore, U.   (2003)   'Localization of ligand-binding sites on human C1q globular head region using recombinant globular head fragments and single-chain antibodies'. Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 1652 (1).  pp. 64 - 74. doi: 10.1016/j.bbapap.2003.08.003 

Kishore, U. , Gupta, SK. , Perdikoulis, MV. , Kojouharova, MS. , et al.   (2003)   'Modular organization of the carboxyl-terminal, globular head region of human C1q A, B, and C chains'. Journal of Immunology, 171 (2).  pp. 812 - 820. 

Shrive, AK. , Tharia, HA. , Strong, P. , Kishore, U. , et al.   (2003)   'High-resolution structural insights into ligand binding and immune cell recognition by human lung surfactant protein D'. Journal of Molecular Biology, 331 (2).  pp. 509 - 523. doi: 10.1016/S0022-2836(03)00761-7 

Kishore, U. , Zhang, W. , Corlett, L. , Waters, P. , et al.   (2003)   'Use of peptide: HLA class II complexes to study specific T cells in autoimmune myasthenia gravis'. Annals of the New York Academy of Sciences, 998 pp. 339 - 342. doi: 10.1196/annals.1254.038 

Singh, M. , Madan, T. , Waters, P. , Parida, SK. , et al.   (2003)   'Protective effects of a recombinant fragment of human surfactant protein D in a murine model of pulmonary hypersensitivity induced by dust mite allergens'. Immunology Letters, 86 (3).  pp. 299 - 307. doi: 10.1016/S0165-2478(03)00033-6 

Plested, CP. , Tang, T. , Spreadbury, I. , Littleton, ET. , et al.   (2002)   'AChR phosphorylation and indirect inhibition of AChR function in seronegative MG'. Neurology, 59 (11).  pp. 1682 - 1688. 

Lu, JH. , Teh, C. , Kishore, U.  and Reid, KBM.   (2002)   'Collectins and ficolins: Sugar pattern recognition molecules of the mammalian innate immune system'. BBA - Biochimica et Biophysica Acta, 1572 (2-3).  pp. 387 - 400. doi: 10.1016/S0304-4165(02)00320-3 

Kishore, U. , Kojouharova, MS.  and Reid, KB.   (2002)   'Recent progress in the understanding of the structure-function relationships of the globular head regions of C1q'. Immunobiology, 205 (4-5).  pp. 355 - 364. doi: 10.1078/0171-2985-00138 

Kishore, U. , Zhang, W. , Corlett, L. , Kelleher, A. , et al.   (2002)   'Use of peptide: HLA class II complexes to study specific T cells in myasthenia gravis'. JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 73 (2).  pp. 229 - 229. 

Perdikoulis, MV. , Kishore, U.  and Reid, KB.   (2001)   'Expression and characterisation of the thrombospondin type I repeats of human properdin'. Biochimica et Biophysica Acta, 1548 (2).  pp. 265 - 277. doi: 10.1016/S0167-4838(01)00238-2 

Kishore, U. , Strong, P. , Perdikoulis, MV.  and Reid, KB.   (2001)   'A recombinant homotrimer, composed of the alpha helical neck region of human surfactant protein D and C1q B chain globular domain, is an inhibitor of the classical complement pathway'. The Journal of Immunology, 166 (1).  pp. 559 - 565. 

Madan, T. , Kishore, U. , Singh, M. , Strong, P. , et al.   (2001)   'Protective role of lung surfactant protein D in a murine model of invasive pulmonary aspergillosis'. Infection and Immunity, 69 (4).  pp. 2728 - 2731. doi: 10.1128/IAI.69.4.2728-2731.2001 

Kishore, U. , Madan, T. , Singh, M.  and Strong, P.   (2001)   'Surfactant proteins A and D protect mice against pulmonary hypersensitivity induced by Aspergillus fumigatus antigens and allergens'. Journal of Clinical Investigation, 107 (4).  pp. 467 - 475. 

Kishore, U.  and Reid, KB.   (2001)   'Structures and functions of mammalian collectins.'. Results and problems in cell differentiation, 33 pp. 225 - 248. 

Aggarwal, NK. , Kishore, U.  and Agarwal, BB.   (2000)   'Hanging-delayed death (a rare phenomenon)'. Medicine, Science and the Law, 40 (3).  pp. 270 - 272. 

Kishore, U.  and Reid, KB.   (2000)   'C1q: structure, function, and receptors'. Immunopharmacology, 49 (1-2).  pp. 159 - 170. doi: 10.1016/S0162-3109(00)80301-X 

Madan, T. , Singh, M. , Saxena, S. , Kishore, U. , et al.   (2000)   'Pulmonary surfactant protein A and D suppress eosinophilia and specific IgE antibodies in a murine model of allergic bronchopulmonary aspergillosis (ABPA)'. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 105 (1).  pp. S301 - S301. doi: 10.1016/S0091-6749(00)91314-9 

Kishore, U.  and Reid, KB.   (1999)   'Modular organization of proteins containing C1q-like globular domain'. Immunopharmacology, 42 (1-3).  pp. 15 - 21. doi: 10.1016/S0162-3109(99)00011-9 

Strong, P. , Kishore, U. , Morgan, C. , Lopez Bernal, A. , et al.   (1998)   'A novel method of purifying lung surfactant proteins A and D from the lung lavage of alveolar proteinosis patients and from pooled amniotic fluid'. Journal of Immunological Methods, 220 (1-2).  pp. 139 - 149. doi: 10.1016/S0022-1759(98)00160-4 

Perdikoulis, MV. , Kishore, U.  and Reid, KBM.   (1998)   'Cloning, expression in E-coli and characterisation of human properdin TSRs.'. MOLECULAR IMMUNOLOGY, 35 (6-7).  pp. 374 - 374. doi: 10.1016/S0161-5890(98)90708-8 

Kishore, U. , Perdikoulis, MV. , Strong, P.  and Reid, KBM.   (1998)   'A homotrimer of the B-chain globular head region of C1q is an inhibitor of C1q-mediated complement activation'. MOLECULAR IMMUNOLOGY, 35 (6-7).  pp. 375 - 375. doi: 10.1016/S0161-5890(98)90710-6 

Leigh, LE. , Ghebrehiwet, B. , Perera, TP. , Bird, IN. , et al.   (1998)   'C1q-mediated chemotaxis by human neutrophils: involvement of gClqR and G-protein signalling mechanisms'. Biochemical Journal, 330 (Pt 1) pp. 247 - 254. 

Kishore, U. , Leigh, LE. , Eggleton, P. , Strong, P. , et al.   (1998)   'Functional characterization of a recombinant form of the C-terminal, globular head region of the B-chain of human serum complement protein, C1q'. Biochemical Journal, 333 (Pt 1) pp. 27 - 32. 

Priyadarsiny, P. , Madan, T. , Kishore, U.  and Sarma, PU.   (1998)   'Role of lung surfactant proteins A & D in host defense against A-fumigatus antigens.'. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 101 (1).  pp. S78 - S78. 

Eggleton, P. , Reid, KB. , Kishore, U.  and Sontheimer, RD.   (1997)   'Clinical relevance of calreticulin in systemic lupus erythematosus'. Lupus, 6 (7).  pp. 564 - 571. doi: 10.1177/096120339700600703 

Madan, T. , Eggleton, P. , Kishore, U. , Strong, P. , et al.   (1997)   'Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages'. Infection and Immunity, 65 (8).  pp. 3171 - 3179. 

Madan, T. , Kishore, U. , Shah, A. , Eggleton, P. , et al.   (1997)   'Lung surfactant proteins A and D can inhibit specific IgE binding to the allergens of Aspergillus fumigatus and block allergen-induced histamine release from human basophils'. Clinical and Experimental Immunology, 110 (2).  pp. 241 - 249. 

Kishore, U. , Eggleton, P.  and Reid, KB.   (1997)   'Modular organization of carbohydrate recognition domains in animal lectins'. Matrix Biology, 15 (8-9).  pp. 583 - 592. doi: 10.1016/S0945-053X(97)90035-4 

Kishore, U. , Sontheimer, RD. , Sastry, KN. , Zappi, EG. , et al.   (1997)   'The systemic lupus erythematosus (SLE) disease autoantigen-calreticulin can inhibit C1q association with immune complexes'. Clinical and Experimental Immunology, 108 (2).  pp. 181 - 190. 

Kishore, U. , Sontheimer, RD. , Sastry, KN. , Zaner, KS. , et al.   (1997)   'Release of calreticulin from neutrophils may alter C1q-mediated immune functions'. Biochemical Journal, 322 (Pt 2) pp. 543 - 550. 

Wang, JY. , Kishore, U. , Lim, BL. , Strong, P.  and Reid, KB.   (1996)   'Interaction of human lung surfactant proteins A and D with mite (Dermatophagoides pteronyssinus) allergens'. Clinical and Experimental Immunology, 106 (2).  pp. 367 - 373. 

Kishore, U. , Wang, J-Y. , Hoppe, H-J.  and Reid, KBM.   (1996)   'The α-helical neck region of human lung surfactant protein D is essential for the binding of the carbohydrate recognition domains to lipopolysaccharides and phospholipids'. Biochemical Journal, 318 (2).  pp. 505 - 511. 

Kishore, U. , Wang, JY. , Hoppe, HJ.  and Reid, KB.   (1996)   'The alpha-helical neck region of human lung surfactant protein D is essential for the binding of the carbohydrate recognition domains to lipopolysaccharides and phospholipids'. Biochemical Journal, 318 (Pt 2) pp. 505 - 511. 

Wang, JY. , Kishore, U.  and Reid, KB.   (1995)   'A recombinant polypeptide, composed of the alpha-helical neck region and the carbohydrate recognition domain of conglutinin, self-associates to give a functionally intact homotrimer'. FEBS Letters, 376 (1-2).  pp. 6 - 10. doi: 10.1016/0014-5793(95)01232-4 

Kishore, U. , Chauhan, M. , Choudhury, NR.  and Khatri, GS.   (1994)   'Overexpression of Escherichia coli single stranded DNA binding protein under bacteriophage T 7 promoter'. Indian Journal of Biochemistry and Biophysics, 31 (4).  pp. 249 - 253. 

Books

Kishore, U.   (2009) Target pattern recognition in innate immunity.  Springer 

Book chapters

Nayak, A. , Kishore, U.  and Bonifati, DM.  (2011)   'Neurodegeneration and neuroinflammation: the active role of complement', in Farooqi, AA. (ed.) Molecular Aspects of Neurodegeneration and Neuroprotection.  Bentham Science Publishers (Forthcoming)  

Waters, P. , Vaid, M. , Kishore, U.  and Madan, T.  (2009)   'SP-A and SP-D as innate immune molecules', in Kishore, U. (ed.) Target Pattern Recognition in Innate Immunity.  Texas, USA Springer-Landes Bioscience 

Conference papers

Gadjeva, M. , Zlatarova, A. , Ruseva, M. , Kishore, U.  and Kojouharova, M.   (2010)   'Mutational analysis of ligand-binding activities of recombinant gC1q heterotrimer' , 23rd International National Complement Workshop. New York, NY. 1 - 5 August. PERGAMON-ELSEVIER SCIENCE LTD, pp. 2260 - 2260. doi: 10.1016/j.molimm.2010.05.188 

Nayak, A. , Waters, P. , Gadjeva, M. , Ferluga, J. , et al.   (2010)   'Recombinant forms of monomeric and homotrimeric globular regions of human C1q as inhibitors of complement and microglia activation induced by beta-amyloid and familial dementia peptides' , 23rd International National Complement Workshop. New York, NY. 1 - 5 August. PERGAMON-ELSEVIER SCIENCE LTD, pp. 2284 - 2285. doi: 10.1016/j.molimm.2010.05.257 

Gadjeva, M. , Ruseva, M. , Zlatarova, A. , Reid, KBM. , et al.   (2007)   'GC1q-ligand interactions - A Ca2+ dependent two-step process' , 11th European Meeting on Complement in Human Disease. Cardiff, WALES. 8 - 11 September. PERGAMON-ELSEVIER SCIENCE LTD, pp. 3983 - 3983. doi: 10.1016/j.molimm.2007.06.192 

Kang, Y. , Kishore, U. , Sim, RB.  and Urban, BC.   (2005)   'Interaction of human C1q and Factor H with apoptotic cells' , 10th Meeting on Complement in Human Disease. Heidelberg, GERMANY. 9 - 13 September. PERGAMON-ELSEVIER SCIENCE LTD, pp. 144 - 144. 

Groeneveld, TWL. , Oroszlan, M. , Faber-Krol, MC. , Bakker, AC. , et al.   (2005)   'Regulation of the inflammatory functions of C1q by the matrix proteoglycan biglycan' , 10th Meeting on Complement in Human Disease. Heidelberg, GERMANY. 9 - 13 September. PERGAMON-ELSEVIER SCIENCE LTD, pp. 152 - 152. 

Trouw, LA. , Seelen, MA. , Groeneveld, TWL. , Duijs, JMGJ. , et al.   (2004)   'Induction of murine lupus-nephritis with an anti-C1q monoclonal antibody' , 20th International Complement Workshop. Honolulu, HI. 13 - 18 June. PERGAMON-ELSEVIER SCIENCE LTD, pp. 319 - 320. 

Gadjeva, M. , Roumenina, L. , Zlatarova, A. , Tsacheva, I. , et al.   (2004)   'Molecular mechanisms of recognition between complement component C1q globular domain and its specific ligands' , 20th International Complement Workshop. Honolulu, HI. 13 - 18 June. PERGAMON-ELSEVIER SCIENCE LTD, pp. 232 - 232. 

Zhang, W. , Leite, I. , Kishore, U. , Water, P.  and Willcox, N.   (2004)   'Use of ELISPOTS to monitor cloning of AChR-specific T-cells from myasthenia gravis patients' , 7th International Congress of the International-Society-of-Neuroimmunology. Venice, ITALY. 28 - 2 October. ELSEVIER SCIENCE BV, pp. 150 - 150. 

Mitchell, DA. , Villiers, CL. , Foster, N. , Kishore, U.  and Sim, RB.   (2004)   'Proteomic profiling of complement-dendritic cell interactions' , 20th International Complement Workshop. Honolulu, HI. 13 - 18 June. PERGAMON-ELSEVIER SCIENCE LTD, pp. 277 - 277. 

Kishore, U. , Madan, T. , Sarma, PU. , Singh, M. , et al.   (2002)   'Protective roles of pulmonary surfactant proteins, SP-A and SP-D, against lung allergy and infection caused by Aspergillus fumigatus' , Workshop on Early Phases of Complement Activation and Innate Immunity. SEEHEIM JUGENHEIM, NAMIBIA. 1 URBAN & FISCHER VERLAG, pp. 610 - 618. doi: 10.1078/0171-2985-00158 

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