Effects of dehydration and hyperthermia on brain and muscle blood flow
Dehydration, the process of losing body water through thermoregulatory sweating, can lead to marked alterations in physiological function and decrements in athletic performance during training and competition in temperate-to-hot environments. Impaired endurance capacity in the dehydrated athlete is often associated with significant cardiovascular strain, typified by gradual reductions in cardiac output, mean arterial pressure and blood flow to exercising limb muscle and skin during prolonged exercise in the heat (Sawka et al. 1979; Montain & Coyle, 1992; González-Alonso et al. 1995, 1998, 2008). Blood flow to the brain might also decline with dehydration since reductions in blood flow to active muscle and skin only account for two-thirds of the fall in cardiac output during prolonged exercise in the heat (González-Alonso et al. 1998). The first study of this project systematically investigated the responses of the cerebral circulation and metabolism to progressive dehydration during submaximal and maximal exercise in the heat and the benefits of maintaining a normal hydration status (euhydration) via fluid ingestion.
A widely held concept in cardiovascular physiology is that blood flow to the muscles of the arm and legs remain unchanged in humans exposed to severe environmental heat stress, despite cardiac output increasing by up to 5-6 L/min compared to control resting conditions (Rowell 1974). This has been interpreted to mean that muscle blood flow regulation is insensitive to increases in tissue temperature. Recent findings from our laboratory, however, challenge this long-standing view by showing that the heat stress-mediated increases in leg blood flow (up to 1.1 L/min above baseline) are accompanied by proportional reductions in muscle blood oxygenation indicative of significant increases in leg muscle blood flow with elevations in temperature (Pearson et al. 2011). Because both blood and muscle temperatures increase rapidly at onset of exercise (González-Alonso et al., 1999; González-Alonso & Calbet 2003), our recent observations raise important questions regarding the role of blood and tissue temperature on the regulation of muscle, brain and systemic blood flow in exercising humans. It is therefore important to further investigate the responses and temperature-sensitive mechanisms controlling limb tissue hyperaemia in the heat stressed human, a second major aim of this project.
This project consisted of 3 different studies, which characterised the impact of hydration and heat stress on brain and active limb muscle blood flow and systemic haemodynamics on regional and systemic metabolism during submaximal and maximal exercise and provided insight into the temperature-sensitive mechanisms controlling local limb tissue blood flow with hyperthermia. Detailed information about the experimental designs employed for each of the studies can be found in the research outputs reported below.
Study 1: Brain circulation and metabolism during prolonged submaximal exercise and incremental maximal exercise with exercise-induced dehydration and hydration.
Study 2: Brain and muscle blood flow and metabolism during incremental cycling exercise with and without heat stress
Study 3: Temperature-sensitive mechanism controlling limb tissue hyperthermia in the heat stressed-human.
Significance and Impact
Knowledge of how vital organs such as the brain, heart and active muscles respond to strenuous exercise is paramount to advance our understanding of exercise fatigue mechanisms and thus optimise human performance. This project characterized the brain, muscle and systemic blood flow and metabolic responses to strenuous submaximal and maximal exercise in conditions commonly experienced by elite and recreational athletes in warm and hot environments. A close association between the development of fatigue and reductions in cerebral and muscle blood flow and oxygen delivery was observed, particularly when fatiguing sooner whilst experiencing significant dehydration and hyperthermia.
Whilst the development of dehydration and hyperthermia exacerbates the cerebrovascular strain and accentuates the fall in cerebral blood flow, the global brain aerobic metabolism is preserved due to compensatory elevations in oxygen and substrate extraction from the circulation. Thus, reduced brain aerobic metabolism is unlikely to contribute to the fall in maximal aerobic power and endurance performance in the dehydrated and hyperthermic athlete. Rather, the early fatigue experienced by the dehydrated athlete in these conditions is coupled to high levels of core hyperthermia and alterations in muscle substrate, glycogen and oxygen utilization and increases in muscle anaerobic metabolism and neural activation, possibly reflecting a mismatch between energy demand and production.
These findings provide physiologists, sport scientists, coaches and athletes with evidence to explain how dehydration and/or hyperthermia impact brain and muscle blood perfusion and metabolism during exercise and heat stress and ultimately human performance. The data further substantiate the use of fluid replacement during exercise as a means of delaying the reductions in brain, skeletal muscle and systemic circulation during exhaustive exercise in temperate to hot environments and thus optimise performance in the endurance athlete.
Trangmar SJ, Chiesa ST, Stock CG, Kalsi KK, Secher NH & González-Alonso J (2014). Dehydration affects cerebral blood flow but not its metabolic rate for oxygen during maximal exercise in trained humans, J Physiol 592, 3143-3160. https://physoc.onlinelibrary.wiley.com/doi/epdf/10.1113/jphysiol.2014.272104
Trangmar SJ, Chiesa ST, Llodio I, Garcia B, Kalsi K, Secher NH & González-Alonso J (2015). Dehydration accelerates reductions in cerebral blood flow during prolonged exercise in the heat without compromising brain metabolism. Am J Physiol Heart Circ Physiol 309,H1598-H1607. https://www.physiology.org/doi/pdf/10.1152/ajpheart.00078.2015
Trangmar SJ, Chiesa ST, Kalsi K, Secher NH & González-Alonso J (2017). Whole body hyperthermia, but not skin hyperthermia, accelerates brain and locomotor limb circulatory strain andimpairs exercise capacity in heat stressed humans. Physiol Rep 5 (2), e13108. https://physoc.onlinelibrary.wiley.com/doi/epdf/10.14814/phy2.13108
Trangmar SJ & González-Alonso J (2017). New insights into the impact of dehydration on blood flow and metabolism during exercise. Exerc Sport Sci Rev 45, 146-153. https://journals.lww.com/acsm-essr/Abstract/2017/07000/New_Insights_Into_the_Impact_of_Dehydration_on.5.aspx
Chiesa ST, Trangmar SJ, Kalsi K, Rakobowchuk M, Banker DS, Lotlikar MD, Ali L & González-Alonso J (2015). Local temperature-sensitive mechanisms are important mediators of limb tissue hysperemia in the heat-stressed human at rest and during small muscle mass exercise. Am J Physiol Heart Circ Physiol 309, H369-H380. https://www.physiology.org/doi/pdf/10.1152/ajpheart.00078.2015
Kalsi KK, Chiesa ST, Trangmar SJ, Lotlikar MD, Ali L, González-Alonso J (2017). Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature-dependent ATP release from human blood and endothelial cells. Exp Physiol 102, 228-244. https://physoc.onlinelibrary.wiley.com/doi/epdf/10.1113/EP085910
González-Alonso J, Calbet JAL, Boushel R, Helge JW, Søndergaard H, Munch-Andersen T, van Hall G, Mortensen SP & Secher NH (2015). Blood temperature and perfusion to exercising and non-exercising human limbs. Exp Physiol 100, 1118-1131. https://physoc.onlinelibrary.wiley.com/doi/epdf/10.1113/EP085383
Kalsi KK & González-Alonso J(2012). Temperature-dependent release of ATP from human erythrocyles: mechanism for the control of local tissue perfusion. Exp Physiol 97, 419-432. https://physoc.onlinelibrary.wiley.com/doi/epdf/10.1113/expphysiol.2011.064238