Christopher Eskiw

Academic qualifications

2004: Ph.D., Programme of Cell Biology, University of Toronto, Toronto, Canada

2001: M.Sc., Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada

1998: B.Sc. Molecular Genetics, Biological Sciences, University of Alberta, Edmonton, Canada

Other responsibilities

2010: Lecturer, Division of Biosciences, School of Health Sciences and Social Care, Brunel University

2007-2010: Post-Doctoral Fellow, Laboratory for Chromatin and Gene Expression, Babraham Institute, Cambridge, UK

2004-2007: Post-Doctoral Fellow, Sir William Dunn School of Pathology, Oxford, UK

Research interests

The aim of my laboratory is to determine how the human genome re-organizes during the ageing process and how this leads to changes in gene expression patterns. We use a wide range of techniques from high resolution imaging (both light and electron microscopy) to molecular biological (chromosome confirmation capture, 3C, assay and high through put RNA sequencing) to determine the functional consequences of the ageing process. We also study cells from Hutchinson-Gilford Progeria Syndrome patients (HGPS) who experience parallel but highly accelerated ageing phenotypes.

Research centres

• Centre for Cell and Chromosome Biology (CCCB)

Publications

Journal Papers

(2012) Eskiw, C., Mitchell, JA., Clay, I., Umlauf, D., Chen, CY., Moir, CA., Eskiw, CH., Schoenfelder, S., Chakalova, L., Nagano, T. and Fraser, P., Nuclear RNA sequencing of the mouse erythroid cell transcriptome, PLoS One 7 (11) : e49274 Download publication

(2011) Eskiw, CH. and Fraser, P., Ultrastructural study of transcription factories in mouse erythroblasts., J Cell Sci 124 (Pt 21) : 3676- 3683 Download publication

(2011) Mehta, IS., Arican, HD., Eskiw, CH., Kill, IR. and Bridger, JM., Farnesyltransferase inhibitor treatment restores chromosome territory positions and active chromosome dynamics in Hutchinson-Gilford Progeria syndrome cells., Genome Biology: biology for the post-genomic era 12 (8) : R74 Download publication

(2011) Mehta, I., Eskiw, C., Aricane, H., Kill, I. and Bridger, J., Farnesyltransferase inhibitor treatment restores chromosome territory positions and active chromosome dynamics in Hutchinson-Gilford Progeria syndrome cells., Genome Biology 12 (8) : R74

(2010) Pink, R., Eskiw, C., Caley, DP. and Carter, D., Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv, PLoS ONE 5 (9) : e13045 Download publication

(2010) Cope, NF., Fraser, P. and Eskiw, CH., The yin and yang of chromatin spatial organization, Genome Biology 11 (3) : 204

(2010) Schoenfelder, S., Sexton, T., Chakalova, L., Cope, NF., Horton, A., Andrews, S., Kurukuti, S., Mitchell, JA., Umlauf, D., Dimitrova, DS., Eskiw, CH., Lou, Y., Wei, C-L., Raun, CY., Bieker, JJ. and Fraser, P., Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells, Nature Genetics 42 (1) : 53- 61

(2009) Eskiw, C. and Fraser, PJ., Inverted rod cells see the light, Nature Cell Biology 11 680- 681

(2008) Carter, DR., Eskiw, C. and Cook, PR., Transcription factories, Biochemical Society Transactions 36 (4) : 585- 589

(2008) Osborne, CS. and Eskiw, CH., Where shall we meet? A role for genome organisation and nuclear sub-compartments in mediating interchromosomal interactions, Journal of Cellular Biochemistry 104 (5) : 1553- 1561

(2008) Eskiw, CH., Rapp, A., Carter, DRF. and Cook, PR., RNA polymerase II activity is located on the surface of protein-rich transcription factories, Journal of Cell Science 121 (12) : 1999- 2007

(2006) Block, GJ., Eskiw, CH., Dellaire, G. and Bazett-Jones, DP., Transcriptional regulation is affected by subnuclear targeting of reporter plasmids to PML nuclear bodies, Molecular and Cellular Biology 26 (23) : 8814- 8825

(2006) Dellaire, G., Eskiw, CH., Dehghani, H., Ching, RW. and Bazett-Jones, DP., Mitotic accumulations of PML protein contribute to the re-establishment of PML nuclear bodies in G1, Journal of Cell Science 119 (6) : 1034- 1042

(2006) Bartlett, J., Blagojevic, J., Carter, D., Eskiw, C., Fromaget, M., Job, C., Shamsher, M., Trindade, IF., Xu, M. and Cook, PR., Specialized transcription factories, Biochemical Society Symposia 73 67- 75

(2005) Ching, R., Dellaire, G., Eskiw, C. and Bazett-Jones, DP., PML bodies: a meeting place for genomic loci?, Journal of Cell Science 118 (5) : 847- 854

(2004) Eskiw, CH., Dellaire, G. and Bazett-Jones, DP., Chromatin contributes to structural integrity of promyelocytic leukemia bodies through a SUMO-1-independent mechanism, Journal of Biological Chemistry 279 (10) : 9577- 9585

(2004) Dellaire, G., Nisman, R., Eskiw, CH. and Bazett-Jones, DP., In situ imaging and isolation of proteins using dsDNA oligonucleotides, Nucleic Acids Research 32 (20) : e165

(2003) Eskiw, CH., Dellaire, G., Mymryk, JS. and Bazett-Jones, DP., Size, position and dynamic behavior of PML nuclear bodies following cell stress as a paradigm for supramolecular trafficking and assembly, Journal of Cell Science 116 (21) : 4455- 4466

(2002) Eskiw, CH. and Bazett-Jones, DP., The promyelocytic leukemia nuclear body: sites of activity?, Biochemistry and Cell Biology 80 (3) : 301- 310

(2001) Ficzycz, A., Eskiw, C., Meyer, D., Marley, KE., Hurt, M. and Ovsenek, N., Expression, activity and subcellular localization of the Yin Yang 1 transcription factor in Xenopus oocytes and embryos, Journal of Biological Chemistry 276 (25) : 22819- 22825

Book Chapters

(2004) Bazett-Jones, DP. and Eskiw, C., Chromatin structure and function: lessons from imaging techniques. In: Zlatanova, J. and Leuba, SH. eds. Chromatin Structure and Dynamics: State-of-the-Art. Elsevier Science