Michael Themis

Academic qualifications

• BSc: 1^st class honours with Brunel University Prize
• PhD: 1994 entitled “Insertional mutagenesis:experiences at the hprt locus”
• Post Graduate Certificate in Learning and Teaching in Higher Education (PGCert)

PhD supervision

The Division of Biosciences is currently offering PhD projects for overseas students who can support their own funding for research. Dr Themis has a number of internationally competitive projects that will provide students who are successful in their applications the opportunity of a sound basis for a career in science relating to gene therapy, stem cell and cancer biology using mouse models of disease, Students are invited to contact Dr Themis by e-mail: michael.themis@brunel.ac.uk to obtain detailed information on the projects on offer.

Other responsibilities

Position history

• 2006 - present: Lecturer Brunel University, Uxbridge (HFCE funded)
• 2006 - present: Honorary Lecturer Imperial College London
• 2005 - 2006: Research Lecturer, Imperial College London. MRC, Wellcome Trust Value In People Award (Welcome Trust and HFCE funded)
• 2000 - 2005: Research Lecturer, Imperial College London (MRC funded)
• 1998 -1999: Senior post-doctoral Research Fellow, Imperial College (MRC funded)
• 1994-1998: Post-doctoral position, St. Mary's Hospital, Paddington, London (March of Dimes funded)

Research centres

• Brunel Institute for Cancer Genetics and Pharmacogenomics (BICGP)
• Centre for Cell and Chromosome Biology (CCCB)
• Centre for Infection, Immunity and Disease Mechanisms (CIIDM)

Membership and affiliation

• Breast Cancer Campaign Scientific Advisory Board
• American Society for Gene Therapy
• Committee member of the ASGT Musculo-Skeletal Disorder Committee. 2004-2007
• European Society for Gene Therapy
• National Translational Cancer Research Network (NTRAC)
• Founder and Associate member of the British Society for Gene Therapy
• Imperial College London Safety Committee

Publications

Journal Papers

(2013) Nowrouzi, A., Cheung, WT., Li, T., Zhang, X., Arens, A., Paruzynski, A., Waddington, SN., Osejindu, E., Reja, S., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, L., Themis, M., Holder, M., Dighe, N., Ruthe, A., Buckley, SM., Bigger, B., Montini, E., Thrasher, AJ., Andrews, R., Roberts, TP., Newbold, RF., Coutelle, C., Schmidt, M. and Themis, M., The Fetal Mouse Is a Sensitive Genotoxicity Model That Exposes Lentiviral-associated Mutagenesis Resulting in Liver Oncogenesis., Mol Ther 21 (2) : 324- 337 Download publication

(2010) David, AL., Abi-Nader, KN., Weisz, B., Shaw, SWS., Themis, M., Cook, T., Coutelle, C., Rodeck, CH. and Peebles, DM., ULTRASONOGRAPHIC DEVELOPMENT OF THE FETAL SHEEP STOMACH AND EVALUATION OF EARLY GESTATION ULTRASOUND-GUIDED IN UTERO I NTRAGASTRIC INJECTION, TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY 49 (1) : 23- 29

(2009) Kennea, NL., Waddington, SN., Chan, J., O'Donoghue, K., Yeung, D., Taylor, DL., Al-Allaf, FA., Pirianov, G., Themis, M., Edwards, AD., Fisk, NM. and Mehmet, H., Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype, Cell Cycle 8 (7) : 1069- 1079

(2008) Buckley, SMK., Waddington, SN., Jezzard, S., Bergau, A., Themis, M., MacVinish, LJ., Cuthbert, AW., Colledge, WH. and Coutelle, C., Intra-amniotic delivery of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred CFTR-knockout mice, Molecular Therapy 16 (5) : 819- 824

(2006) David, A., Weisz, B., Ruthe, A., Peebles, DM., Coutelle, C., Rodeck, CH. and Themis, M., Gene transfer to human fetal tissues in vitro, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY 113 (7) : 863- 863

(2006) David, AL., Peebles, DM., Gregory, L., Waddington, SN., Themis, M., Weisz, B., Ruthe, A., Lawrence, L., Cook, T., Rodeck, CH. and Coutelle, C., Clinically applicable procedure for gene delivery to fetal gut by ultrasound-guided gastric injection: Toward prenatal prevention of early-onset intestinal diseases, HUMAN GENE THERAPY 17 (7) : 767- 779

(2006) Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, LG., Nivsarkar, M., Holder, MV., Buckley, SMK., Dighe, N., Ruthe, AT., Mistry, A., Bigger, B., Rahim, A., Nguyen, TH., Trono, D., Thrasher, AJ. and Coutelle, C., Erratum: "Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice" (Molecular Therapy (2005) vol. 12 (763-771) 10.1016/j.ymthe.2005.07.358), Molecular Therapy 13 (4) : 830- 830

(2006) Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, LG., Nivsarkar, M., Themis, M., Holder, MV., Buckley, SMK., Dighe, N., Ruthe, AT., Mistry, A., Bigger, B., Rahim, A., Nguyen, TH., Trono, D., Thrasher, AJ. and Coutelle, C., Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice (vol 12, pg 763, 2005), MOLECULAR THERAPY 13 (4) : 830- 830

(2006) Tolmachov, O., Ma, YL., Themis, M., Patel, P., Spohr, H., Macleod, KT., Ullrich, ND., Kienast, Y., Coutelle, C. and Peters, NS., Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro., BMC Cardiovasc Disord 6 25- Download publication

(2006) Bigger, BW., Siapati, EK., Mistry, A., Waddington, SN., Nivsarkar, MS., Jacobs, L., Perrett, R., Holder, MV., Ridler, C., Kemball-Cook, G., Ali, RR., Forbes, SJ., Coutelle, C., Wright, N., Alison, M., Thrasher, AJ., Bonnet, D. and Themis, M., Permanent partial phenotypic correction and tolerance in a mouse model of hemophilia B by stem cell gene delivery of human factor IX., Gene Therapy 13 (2) : 117- 126

(2006) David, AL., Weisz, B., Gregory, L., Themis, M., Cook, T., Roubliova, X., Deprest, J., Coutelle, C., Rodeck, CH. and Peebles, DM., Ultrasound-guided injection and occlusion of the trachea in fetal sheep, ULTRASOUND IN OBSTETRICS & GYNECOLOGY 28 (1) : 82- 88

(2005) Buckley, SM., Waddington, SN., Jezzard, S., Lawrence, L., Schneider, H., Holder, MV., Themis, M. and Coutelle, C., Factors influencing adenovirus-mediated airway transduction in fetal mice, Molecular Therapy 12 (3) : 484- 492

(2005) Waddington, SN., Kramer, MG., Hernandez-Alcoceba, R., Buckley, SM., Themis, M., Coutelle, C. and Prieto, J., In utero gene therapy: Current challenges and perspectives, Molecular Therapy 11 (5) : 661- 676

(2005) Al-Allaf, F., Tolmachov, O., Themis, M. and Coutelle, C., Accurate size gauging of ExoIII/S1-generated deletions by PCR analysis of ligation mixtures., Anal Biochem 339 (2) : 348- 350 Download publication

(2005) Siapati, EK., Bigger, BW., Miskin, J., Chipchase, D., Parsley, KL., Mitrophanous, K., Themis, M., Thrasher, AJ. and Bonnet, D., Comparison of HIV- and EIAV-based vectors on their efficiency in transducing murine and human hematopoietic repopulating cells, MOLECULAR THERAPY 12 (3) : 537- 546

(2005) Al-Allaf, FA., Tolmachov, O., Themis, M. and Coutelle, C., Coupled analysis of bacterial transformants and ligation mixture by duplex PCR enables detection of fatal instability of a nascent recombinant plasmid., J Biochem Biophys Methods 64 (2) : 142- 146 Download publication

(2005) Coutelle, C., Themis, M., Waddington, SN., Buckley, SMK., Gregory, LG., Nivsarkar, MS., David, AL., Peebles, D., Weisz, B. and Rodeck, C., Gene therapy progress and prospects: Fetal gene therapy - first proofs of concept - some adverse effects, GENE THERAPY 12 (22) : 1601- 1607

(2005) Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., Gregory, LG., Nivsarkar, M., Themis, M., Holder, MV., Buckley, SM., Dighe, N., Ruthe, AT., Mistry, A., Bigger, B., Rahim, A., Nguyen, TH., Trono, D., Thrasher, AJ. and Coutelle, C., Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice., Molecular Therapy 12 (4) : 763- 771

(2005) Weisz, B., David, AL., Gregory, LG., Perocheau, D., Ruthe, A., Waddington, SN., Themis, M., Cook, T., Coutelle, C., Rodeck, CH. and Peebles, DM., Targeting the respiratory muscles of fetal sheep for prenatal gene therapy for Duchenne muscular dystrophy, AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY 193 (3) : 1105- 1109

(2005) Weisz, B., David, AL., Gregory, LG., Perocheau, D., Ruthe, A., Waddington, SN., Themis, M., Cook, T., Coutelle, C., Rodeck, CH. and Peebles, DM., Targeting the respiratory muscles of fetal sheep for prenatal gene therapy for Duchenne muscular dystrophy, American Journal of Obstetrics and Gynecology 193 (3 SUPPL.) : 1105- 1109

(2004) Waddington, SN., Nivsarkar, MS., Mistry, AR., Buckley, SMK., Kemball-Cook, G., Mosley, KL., Mitrophanous, K., Radcliffe, P., Holder, MV., Brittan, M., Georgiadis, A., Al-Allaf, F., Bigger, BW., Gregory, LG., Cook, HT., Ali, RR., Thrasher, A., Tuddenham, EGD., Themis, M. and Coutelle, C., Permanent phenotypic correction of hemophilia B in immunocompetent mice by prenatal gene therapy, BLOOD 104 (9) : 2714- 2721

(2004) Gregory, LG., Waddington, SN., Holder, MV., Mitrophanous, KA., Buckley, SM., Mosley, KL., Bigger, BW., Ellard, FM., Walmsley, LE., Lawrence, L., Al-Allaf, F., Kingsman, S., Coutelle, C. and Themis, M., Highly efficient EIAV-mediated in utero gene transfer and expression in the major muscle groups affected by Duchenne muscular dystrophy, Gene Therapy 11 (14) : 1117- 1125

(2004) Waddington, SN., Buckley, SM., Bernloehr, C., Bossow, S., Ungerechts, G., Cook, T., Gregory, L., Rahim, A., Themis, M., Neubert, WJ., Coutelle, C., Lauer, UM. and Bitzer, M., Reduced toxicity of F-deficient Sendai virus vector in the mouse fetus, Gene Ther 11 (7) : 599- 608

(2004) Mellor, N., Themis, M., Selden, C., Jones, M. and Hodgson, HJF., Characteristics of murine histidinaemia and its potential for genetic manipulation, LIVER INTERNATIONAL 24 (4) : 354- 360

(2004) Alison, MR., Vig, P., Russo, F., Bigger, BW., Amofah, E., Themis, M. and Forbes, S., Hepatic stem cells: from inside and outside the liver?, CELL PROLIFERATION 37 (1) : 1- 21

(2004) Peebles, D., Gregory, LG., David, A., Themis, M., Waddington, SN., Knapton, HJ., Miah, M., Cook, T., Lawrence, L., Nivsarkar, M., Rodeck, C. and Coutelle, C., Widespread and efficient marker gene expression in the airway epithelia of fetal sheep after minimally invasive tracheal application of recombinant adenovirus in utero, GENE THERAPY 11 (1) : 70- 78

(2003) Themis, M., May, D., Coutelle, C. and Newbold, RF., Mutational effects of retrovirus insertion on the genome of V79 cells by an attenuated retrovirus vector: Implications for gene therapy, Gene Therapy 10 (19) : 1703- 1711

(2003) Waddington, SN., Mitrophanous, KA., Ellard, FM., Buckley, SM., Nivsarkar, M., Lawrence, L., Cook, HT., Al-Allaf, F., Bigger, B., Kingsman, SM., Coutelle, C. and Themis, M., Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice, Gene Therapy 10 (15) : 1234- 1240

(2003) Waddington, SN., Buckley, SMK., Nivsarkar, M., Jezzard, S., Schneider, H., Dahse, T., Kemball-Cook, G., Miah, M., Tucker, N., Dallman, MJ., Themis, M. and Coutelle, C., In utero gene transfer of human factor IX to fetal mice can induce postnatal tolerance of the exogenous clotting factor, BLOOD 101 (4) : 1359- 1366

(2003) Selden, C., Casbard, A., Themis, M. and Hodgson, HJF., Characterization of long-term survival of syngeneic hepatocytes in rat peritoneum, CELL TRANSPLANTATION 12 (6) : 569- 578

(2003) Gregory, LG., Harbottle, RP., Lawrence, L., Knapton, HJ., Themis, M. and Coutelle, C., Enhancement of adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in vivo., Mol Ther 7 (1) : 19- 26 Download publication

(2003) David, AL., Peebles, DM., Gregory, L., Themis, M., Cook, T., Coutelle, C. and Rodeck, CH., Percutaneous ultrasound-guided injection of the trachea in fetal sheep: A novel technique to target the fetal airways, FETAL DIAGNOSIS AND THERAPY 18 (5) : 385- 390

(2003) David, A., Cook, T., Waddington, S., Peebles, D., Nivsarkar, M., Knapton, H., Miah, M., Dahse, T., Noakes, D., Schneider, H., Rodeck, C., Coutelle, C. and Themis, M., Ultrasound-guided percutaneous delivery of adenoviral vectors encoding the beta-galactosidase and human factor IX genes to early gestation fetal sheep in utero, HUMAN GENE THERAPY 14 (4) : 353- 364

(2002) Eden, ER., Patel, DD., Sun, XM., Burden, JJ., Themis, M., Edwards, M., Lee, P., Neuwirth, C., Naoumova, RP. and Soutar, AK., Restoration of LDL receptor function in cells from patients with autosomal recessive hypercholesterolemia by retroviral expression of ARH1, JOURNAL OF CLINICAL INVESTIGATION 110 (11) : 1695- 1702

(2001) Chan, LMC., Coutelle, C. and Themis, M., A novel human suspension culture packaging cell line for production of high-titre retroviral vectors, GENE THERAPY 8 (9) : 697- 703

(2001) Tolmachov, O., Themis, M., Poznansky, M., Coutelle, C. and Peters, N., Construction of connexin 43 transducing retrovirus to produce electrocompetent skeletal muscle tissue for transplantation into the damaged heart, EUROPEAN HEART JOURNAL 22 429- 429

(2000) Schneider, H., Groves, M., Mühle, C., Reynolds, PN., Knight, A., Themis, M., Carvajal, J., Scaravilli, F., Curiel, DT., Fairweather, NF. and Coutelle, C., Retargeting of adenoviral vectors to neurons using the Hc fragment of tetanus toxin, Gene Therapy 7 (18) : 1584- 1592

(2000) Forbes, SJ., Themis, M., Alison, MR., Shiota, A., Kobayashi, T., Coutelle, C. and Hodgson, HJ., Tri-iodothyronine and a deleted form of hepatocyte growth factor act synergistically to enhance liver proliferation and enable in vivo retroviral gene transfer via the peripheral venous system, Gene Therapy 7 (9) : 784- 789

(2000) McKay, TR., MacVinish, LJ., Carpenter, B., Themis, M., Jezzard, S., Goldin, R., Pavirani, A., Hickman, ME., Cuthbert, AW. and Coutelle, C., Selective in vivo transfection of murine biliary epithelia using polycation-enhanced adenovirus, Gene Therapy 7 (8) : 644- 652

(2000) Forbes, SJ., Themis, M., Alison, MR., Sarosi, I., Coutelle, C. and Hodgson, HJ., Synergistic growth factors enhance rat liver proliferation and enable retroviral gene transfer via a peripheral vein, Gastroenterology 118 (3) : 591- 598

(2000) Mellor, N., Themis, M., Selden, C., Jones, M. and Hodgson, HJ., Amelioration of murine histidinaemia using retroviral gene transfer., HEPATOLOGY 32 (4) : 182A- 182A

(1999) Mellor, N., Themis, M., Selden, C. and Hodgson, HJ., An in-vitro model of histidinemia ameliorated by a histidase encoding retrovirus., HEPATOLOGY 30 (4) : 514A- 514A

(1999) Forbes, SJ., Fajinmi, MA., Themis, M., Alison, MR., Coutelle, C. and Hodgson, HH., Gene transfer to the carbon tetrachloride damaged rat liver in vivo with adenoviral and retroviral based vectors., HEPATOLOGY 30 (4) : 514A- 514A

(1999) Themis, M., Schneider, H., Kiserud, T., Cook, T., Adebakin, S., Jezzard, S., Forbes, S., Hanson, M., Pavirani, A., Rodeck, C. and Coutelle, C., Successful expression of beta-galactosidase and factor IX transgenes in fetal and neonatal sheep after ultrasound-guided percutaneous adenovirus vector administration into the umbilical vein, GENE THERAPY 6 (7) : 1239- 1248

(1999) Schneider, H., Adebakin, S., Themis, M., Cook, T., Douar, AM., Pavirani, A. and Coutelle, C., Therapeutic plasma concentrations of human factor IX in mice after gene delivery into the amniotic cavity: A model for the prenatal treatment of haemophilia B, JOURNAL OF GENE MEDICINE 1 (6) : 424- 432

(1998) Themis, M., Forbes, SJ., Chan, L., Cooper, RG., Etheridge, CJ., Miller, AD., Hodgson, HJ. and Coutelle, C., Enhanced in vitro and in vivo gene delivery using cationic agent complexed retrovirus vectors, Gene Therapy 5 (9) : 1180- 1186

(1998) Forbes, SJ., Themis, M., Alison, MR., Selden, C., Coutelle, C. and Hodgson, HJF., Retroviral gene transfer to the liver in vivo during tri-iodothyronine induced hyperplasia, GENE THERAPY 5 (4) : 552- 555

(1997) Douar, AM., Adebakin, S., Themis, M., Pavirani, A., Cook, T. and Coutelle, C., Foetal gene delivery in mice by intra-amniotic administration of retroviral producer cells and adenovirus, Gene Therapy 4 (9) : 883- 890

(1996) Douar, AM., Themis, M. and Coutelle, C., Fetal somatic gene therapy, Molecular Human Reproduction 2 (9) : 633- 641

(1996) Douar, AM., Themis, M., Sandig, V., Friedmann, T. and Coutelle, C., Effect of amniotic fluid on cationic lipid mediated transfection and retroviral infection, GENE THERAPY 3 (9) : 789- 796

(1995) Trott, DA., Cuthbert, AP., Todd, CM., Themis, M. and Newbold, RF., Novel use of a selectable fusion gene as an "in-out" marker for studying genetic loss in mammalian cells, Molecular Carcinogenesis 12 (4) : 213- 224

(1995) Cuthbert, AP., Trott, DA., Ekong, RM., Jezzard, S., England, NL., Themis, M., Todd, CM. and Newbold, RF., Construction and characterization of a highly stable human: rodent monochromosomal hybrid panel for genetic complementation and genome mapping studies., Cytogenet Cell Genet 71 (1) : 68- 76 Download publication

Conference Papers

(2006) Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, YH., Al-Allaf, F., Gregory, L., Nivsarkar, M., Themis, M., Holder, M., Buckley, SMK., Dighe, N., Ruthe, A., Mistry, A., Bigger, B., Thrasher, A. and Coutelle, C., Oncogenesis following delivery of a non-primate lentiviral gene therapy vector to fetal mice, Autumn Meeting of the British-Toxicology-Society, TOXICOLOGY (219) : 233- 233

(2005) Bigger, BW., Waddington, SN., Jones, N., Buch, M., Jacobs, L., Perrett, R., Nivsarkar, M., Watt, SM., Wood, K. and Themis, M., Non-myeloablative stem cell gene delivery effectively establishes long-term gene expression and mixed chimerism in immunocompetent mice, 34th Annual Meeting of the International-Society-for-Experimental-Hematology, EXPERIMENTAL HEMATOLOGY (33) : 41- 42

(2005) Themis, M., Bennett, PR., Mehmet, H., Waddington, SN. and Buckley, SM., The development of a mouse model to study the effect of uterine infection on preterm labour and fetal outcome., 52nd Annual Meeting of the Society-for-Gynecologic-Investigation, JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION (12) : 259A- 259A

(2004) Themis, M., An in vitro hprt locus insertion mutagenesis assay, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S37- S37

(2004) Bergau, A., Gregory, L., Waddington, S., Harbottle, R., Lawrence, L., Coutelle, C. and Themis, M., Comparative analysis of the human cytokeratin K18 promoter with the CMV promoter by hydrodynamic injection of adult MF-1 mice, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S5- S6

(2004) Holder, MV., Waddington, SN., Gregory, LG., Kramer, MG., Prieto, J., Mitrophanous, K., Kingsman, S., Coutelle, C., Themis, M. and Bigger, BW., Comparison of gene expression from liver specific promoter/enhancer combinations in the context of a lentiviral backbone, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S9- S9

(2004) Waddington, SN., Kennea, NL., Buckley, SMK., Gregory, LG., Themis, M. and Coutelle, C., Fetal and neonatal gene therapy: benefits and pitfalls, 2nd European Conference and Practical Course on Towards Clinical Gene Therapy - Preclinical Gene Transfer Assessment, GENE THERAPY (11) : S92- S97

(2004) Ma, YI., Tolmachov, O., Themis, M., Spohr, H., Kienast, Y., Coutelle, C. and Peters, NS., Field action potential activation and propagation in cocultured cardiac myocytes and Cx43 transduced skeletal myoblasts in a multi-electrode array, Annual Conference of the British-Cardiac-Society, HEART (90) : A37- A37

(2004) Chan, J., O'Donoghue, K., Kennea, N., de la Fuente, J., Waddington, S., Themis, M., Mehmet, H., Watt, D., Morgan, J. and Fisk, N., Human fetal mesenchymal stem cells for intrauterine cellular therapy, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S17- S17

(2004) Bigger, BW., Siapati, KE., Mistry, A., Waddington, SN., Nivsarkar, MS., Holder, MV., Ridler, C., Kemball-Cook, G., Ali, RR., Forbes, S., Coutelle, C., Alison, M., Wright, N., Thrasher, AJ., Bonnet, D. and Themis, M., Induction of immune tolerance and partial phenotypic correction of haermophiliac mice by lentiviral gene transfer of human factor IX to haematopoietic stem cells, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S15- S15

(2004) Bigger, BW., Siapati, EK., Mistry, A., Waddington, SN., Nivsarkar, MS., Holder, MV., Ridler, C., Kemball-Cook, G., Ali, RR., Forbes, S., Coutelle, C., Wright, N., Alison, M., Thrasher, A., Bonnet, D. and Themis, M., Induction of immune tolerance and phenotypic rescue of hemophilic mice by stem cell mediated gene transfer, 7th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (9) : S64- S64

(2004) Waddington, SN., Nivsarkar, M., Mistry, A., Buckley, SMK., Kemball-Cook, G., Mosley, KL., Mitrophanous, K., Radcliffe, P., Holder, M., Brittan, M., Georgiadis, T., Al-Allaf, F., Bigger, B., Gregory, L., Cook, HT., Ali, RR., Thrasher, A., Tuddenham, EGD., Themis, M. and Coutelle, C., Permanent phenotypic correction of Haemophilia B in immunocompetent mice by prenatal gene therapy, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S17- S17

(2004) Gregory, LG., Waddington, SN., Holder, M., Mitrophanous, KA., Buckley, SMK., Ellard, FM., Walmsley, LE., Wells, D., Lui, K., Kingsman, S., Coutelle, C. and Themis, M., Pseudotyped lentiviral vectors: Toward an applicability for in utero treatment of Duchenne Muscular Dystrophy, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S18- S18

(2004) Weisz, B., David, AL., Peebles, DM., Gregory, L., Perocheau, D., Themis, M., Terry, C., Coutelle, C. and Rodeck, CH., Targeting the fetal gut for prenatal gene therapy., 51st Annual Meeting of the Society-for-Gynecologic-Investigation, JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION (11) : 182A- 182A

(2004) Weisz, B., David, A., Peebles, D., Gregory, L., Perocheau, D., Waddington, S., Themis, M., Cook, T., Coutelle, C. and Rodeck, C., Targeting the fetal respiratory muscles for prenatal gene therapy for Duchenne muscular dystrophy, 25th Annual Meeting of the Society-for-Maternal-Fetal-Medicine, AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY (191) : S22- S22

(2004) Nivsarkar, MS., Nathwani, A., Mosley, K., Davies, N., Gregory, LG., David, AL., Themis, M., Coutelle, C. and Waddington, SN., Testosterone induced increase in transgene expression in vivo using Adenoviral Gene Delivery Vectors, 1st Annual Conference of the British-Society-for-Gene-Therapy, JOURNAL OF GENE MEDICINE (6) : S12- S13

(2004) Themis, M., Gregory, LG., Waddington, SN., Holder, MV., Mitrophanous, KA., Buckley, SMK., Bigger, BW., Ellard, FE., Walmsley, LE., Radcliff, P., Mazarakis, N., Azzouz, M., Lawrence, L., Cook, T., Allaf, FA., Kingsman, S. and Coutelle, C., The fetal approach: A novel therapy for the treatment of musculo-skeletal disease, 7th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (9) : S91- S91

(2004) David, A., Weisz, B., Peebles, D., Roubliova, X., Perocheau, D., Themis, M., Cook, T., Coutelle, C., Deprest, J. and Rodeck, C., Ultrasound guided placement of an occlusive balloon in the trachea of fetal sheep, 25th Annual Meeting of the Society-for-Maternal-Fetal-Medicine, AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY (191) : S166- S166

(2003) Waddington, SN., Buckley, SMK., Cook, T., Gregory, L., Rahim, A., Nivsarkar, M., Themis, M., Bernloehr, C., Neubert, WJ., Lauer, UM., Coutelle, C. and Bitzer, M., Fetal gene delivery using first and second generation Sendai virus, 6th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (7) : S4- S4

(2003) Themis, M., Waddington, S., Holder, M., Buckley, S., Mitrophanos, K., Bigger, B., Lawrence, L., Cook, T., Allaf, F., Kingsman, S., Coutelle, C. and Gregory, L., Highly efficient and comprehensive gene transfer to all muscle groups necessary for the potential treatment of Duchenne/Becker muscular dystrophies following in utero delivery of a lentivirus vector in immuno-competent mice, 6th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (7) : S320- S320

(2003) Buckley, SMK., Waddington, SN., Jezzard, S., Themis, M., Colledge, WH. and Coutelle, C., Intra-amniotic application of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred Cftr-knockout mice, 6th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (7) : S200- S200

(2003) Azzouz, M., Le, TN., Waddington, S., Walmsley, L., Monani, U., Wilkes, FJ., Themis, M., Mitrophanous, KA., Burghes, A. and Mazarakis, ND., Lentiviral vector-mediated gene therapy for a mouse model of spinal muscular atrophy, 6th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (7) : S248- S248

(2003) Coutelle, C., Themis, M., Waddington, S., Gregory, L., Nivsarkar, M., Buckley, S., Cook, T., Rodeck, C., Peebles, D. and David, A., The hopes and fears of in utero gene therapy for genetic disease--a review., , Placenta (24 Suppl B) : S114- S121 Download publication

(2003) Gregory, LG., Peebles, D., David, A., Themis, M., Waddington, SN., Knapton, HJ., Miah, M., Terry, C., Lawrence, L., Nivsarkar, M., Rodeck, C. and Coutelle, C., Widespread and efficient adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in adult mice and fetal sheep after minimally invasive tracheal application, 6th Annual Meeting of the American-Society-of-Gene-Therapy, MOLECULAR THERAPY (7) : S142- S143

(2002) Ma, YL., Tolmachov, O., Themis, M., Poznansky, M., Coutelle, C. and Peters, NS., Enhanced gap-junctional coupling in connexin43 transduced skeletal myoblasts, American-Heart-Association Abstracts From Scientific Sessions, CIRCULATION (106) : 281- 281

(2002) Ma, Y., Tolmacho, O., Themis, M., Poznansky, M., Coutelle, C. and Peters, NS., Intercellular communication in connexin43 transfected skeletal myocytes, Congress of the European-Society-of-Cardiology, EUROPEAN HEART JOURNAL (23) : 42- 42

(2001) Coutelle, C., Themis, M., Schneider, H., Kiserud, I., Cook, T., Douar, AM., Hanson, M., Pavirani, A., Rodeck, C., Holzgreve, W. and Lessl, M., Fetal somatic gene therapy - A preventive approach to the treatment of genetic disease: The case for, 33rd Ernst-Schering-Research-Foundation Workshop, STEM CELLS FORM CORD BLOOD, IN UTERO STEM CELL DEVELOPMENT, AND TRANSPLANTATION-INCLUSIVE GENE THERAPY (33) : 99- 114