Dr Uday Kishore, PhD

Academic qualifications

• PhD (1995): Department of Zoology, University of Delhi and CSIR Institute of Genomics and Integrative Biology, Delhi, Indi
  Thesis: Chemical gene synthesis and expression of Human Epidermal Growth Factor in Escherichia coli.

Profile

I studied biology in the school, did B.Sc. in Zoology and M.Sc. in Cell Biology, and went on to carry out PhD research in molecular biology from Delhi. After a short stint at Salk Institute, San Diego as a NASA fellow, I joined in 1996 Department of Biochemistry, Oxford, first as a Wellcome Trust Overseas Fellow, and then as a Research Associate within Medical Research Council Immunochemistry Unit. At Oxford, I became interested in pattern recognizing innate immune molecules such as complement protein C1q and pulmonary surfactant proteins SP-A and SP-D. The identification of a major role of SP-D in controlling pulmonary inflammation and infection by our group took me further into host-pathogen interaction. I became involved in a range of projects that culminated in demonstrating that recombinant SP-D can have therapeutic potential again pulmonary hypersensitivity and invasive infection. Since then, I have been concentrating on clinical aspects of the above mentioned proteins that have ramifications as far as asthma, infection, autoimmunity, transplantation and a number of neonatal conditions.

My current interest lies in role of innate immunity in host defence using a model fungal pathogen, Aspergillus fumigatus. This pathogen causes allergic bronchopulmonary aspergillosis (ABPA) in immunocompetent individuals, while being capable of causing fatal invasive aspegillosis in immunocompromised subjects. Human SP-D is a pulmonary surfactant-associated innate immune molecule. Our studies have established its potent anti-microbial and anti-inflammatory properties in vitro, in vivo and ex vivo. Using a murine model of severe lung infection (invasive pulmonary aspergillosis, IPA)  caused by a fungal pathogen, Aspergillus fumigatus (Afu), we found that treatment with rhSP-D can rescue mice from certain death. All untreated IPA mice died within 5-7 days, the rhSP-D treated group showed >90% survival (without any side effects). The SP-D mediated protective mechanisms include agglutination of pathogen, fungistasis, enhanced killing by phagocytes and predominant Th1 response. Increased susceptibility of SP-D gene deficient mice to IPA supports our view. Patients, who are immunocompromised or immunodeficient due to chemotherapy, cancer, transplantation or AIDS are high risk IPA groups.

Using animal models of asthma caused by Afu and dust mite, we have shown that rhSP-D has a long term therapeutic value in dampening asthmatic symptoms in mice. The therapeutic mechanisms include inhibition of allergen-IgE binding and histamine release by sensitized mast cells, dramatic downregulation of specific IgG and IgE antibodies and pulmonary and peripheral eosinophilia, a shift from pathogenic Th2 to a protective Th1 cytokine response, inhibition of mechanisms that cause airway remodelling, and apoptosis induction in sensitised eosinophils. This is consistent with inherent hypereosinophilia and Th2 predominance, and hence increased susceptibility of SP-D gene deficient mice, to allergic hypersensitivity. Thus, another important milestone in sight is to evaluate therapeutuc efficacies of a recombinant form of human SP-D in patients suffering from asthma and lung infection. I aim to further understand how SP-D modulates immune responses, and how it maintains persistence of its therapeautic effect. Using animal models of ABPA and IPA in wild type and SP-D knock out mice, we will try to understand how SP-D modulates eosinophils, dendritic cells (DC), and helper T cell polarisation to protective Th1 response that are so central to therapeutic mechanisms.

In addition, I remain interested in structural and functional aspects of the globular head region of human C1q, the first subcomponent of the classical complement pathway. The potential application of the recombinant forms of C1q globular head domains in dampening complement activation in a range of inflammatory conditions including neurodegenerative diseseaes  is also being explored on my laboratory. A number of above mentioned studies have led to setting up of clinical trials, which remain at the core of my translational research.

Committee responsibilities

Biosciences Research Committee

Admin responsibilities

Module co-ordinator:

BB2405 Genetic Engineering and Immunobiology;

BB3216 Microbial Pathogenesis

Deputy Postgraduate Admission Tutor, Biosciences

Director, Centre for Infection, Immunity and Disease Mechanisms

Other responsibilities

Personal tutor to a number of undergraduate and postgraduate students

Teaching responsibilities

BB2405 Immunobiology

BB2216 Medical Microbiology

BB3216 Microbial Pathogenesis

BB3320 Medical Immunology

PhD supervision

The Kishore Group:

Annapurna Nayak: Role of C1q in neuroinflammation and neurodegeneration associated with Alzheimer's disease and familial dementia

Asif Qaseem: Understanding immunological mechanisms involved in the control of asthma by surfactant protein SP-D

Najmunisa Karbani: Examining roles of SP-A and SP-D in foetal-maternal interaction

Lina Pednekar: Involvement of C1q and C1q receptors in drug-induced susceptibility of tumours to complement attack

Priyaa Madhukaran: Interaction of SP-A and SP-D with decidual macrophages and natural killer cells during pregnancy

Eswari Marri: Interaction of SP-A and SP-D with immune cells involved in allergic hypersensitivity

Agnes Boateng: Innate immunity and cancer

Dr Abhishek Shastri: Immunological basis of neuroinflammation and neurodegeneration

Aline Lokoto: Host-pathogen interaction in Schistosomiasis

Research interests

1. Mechanisms underlying therapeutic effects of exogenous recombinant surfactant protein D (SP-D) in disease models of pulmonary hypersensitivity, inflammation and infection

2. Host pathogen interaction studies using models such as Aspergillus fumigates, Mycobacterium tuberculosis, malaria parasite and Schistosomes.

3. In vivo modulation of dendritic cell functions and mechanism of helper T cell polarisation by SP-D

4. Mechanisms governing SP-D mediated survival and induction of apoptosis of eosinophils and macrophages

5. Instructive role of collectins and complement proteins in dendritic cell-mediated handling of apoptotic cells

6. Structure-function studies on complement protein C1q and novel members of C1q/TNF super family

7. Generation of recombinant inhibitors of classical complement pathway

8. Role of complement in Alzheimer’s disease and familial dementia

9. Immunological nature of fetal-maternal interaction; involvement of surfactant proteins in parturition, pre-term birth, preeclampsia, miscarriage and intrauterine growth retardation

10.  Innate immune aspects of cancer

11. Identification, cloning, characterisation and tolerisation of pathogenic T cells in autoimmunity using MHC class II (HLA-DR) multimer technology.  

We have recently established a protective role of SP-D in asthma and lung infection. Using an opportunistic fungal pathogen, Aspergillus fumigatus, we have also shown that SP-D treatment can be very effective in curing allergic as well as invasive (infective) aspergillosis. It is becoming increasingly clear that A. fumigatus has become a serious health concern especially in the immunocompromised or immunosuppressed individuals and there is a requirement for novel therapies against fungal infection in view of toxicity associated with current drugs and emerging cases of drug resistant strains. In this context, lung surfactant protein D (SP-D) based therapy has an excellent potential for research and development. Our research has opened avenues for further assessment of the therapeutic use of recombinant SP-D in pulmonary hypersensitivity, inflammation and infection. We are in the process of initiating Phase I clinical trial.  

Our laboratory research relies on a range of techniques and strategies including gene synthesis, cDNA cloning, recombinant protein expression in E. coli, Pichia pastoris, baculovirus, Drosophila S2 cells, mammalian cell line (HEK, CHO and COS), protein purification, site-directed mutagenesis, structure-function studies, DNA-protein and protein-protein interactions, murine models of lung allergy, inflammation, oedema and infection, immunoassays, FACS, in vitro and ex vivo cell culture, proteomics, microarray, and various microscopic techniques.  

We also have a number of collaborations in the field of protein structure (crystallography and NMR), in vivo inhibition of complement pathways, role of classical complement pathway in lupus nephritis, involvement of classical and lectin complement pathways during kidney ischemia reperfusion, induction of T cell tolerance by soluble HLA-DR, mechanism of growth inhibition by SP-D using Listeria monocytogens and Aspergillus fumigatus as microbial targets, and fetal-maternal immunity.

Research centres

• Centre for Infection, Immunity and Disease Mechanisms (CIIDM)

Membership and affiliation

International Complement Society

European Complement Society

Complement UK

Association of Clinical Biochemists

Professional activities or recognition

Pre-docstoral positions:

• 1995: NASA Fellow at the Salk Institute for Biological Studies, La Jolla, California, U.S.A.
• Nov, 1994-April, 1995: Visiting Student at the MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, UK.
• 1990-94: Research Fellow at the CSIR Institute of Genomics and Integrative Biology, Delhi and Department of Zoology, University of Delhi.
• 1986-88: Research Assistant at the CSIR Institute of Genomics and Integrative Biology, Delhi. 

Post-dosctoral positions:

• Since May 2006: University Lecturer in Immunology at the Division of Biosciences, School of Health Sciences and Social Care, Brunel University, Uxbridge Campus, West London, UK.
• 2004-2005: Alexander von Humboldt International fellow at the Institute of Medical Microbiology, Justus-Leibig-University, Giessen, Germany.
• 1999-2003: European Commission Research Scientist at the Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.
• 1997-98: Research Associate at the MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, UK.
• 1996: Wellcome Trust International Fellow at the Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, UK.

Awards/distinctions:

Alexander von Humboldt International Fellow, 2005

European Commission Young Scientist Award, 2002

• MRC Investigator Award, 1998

Wellcome International Fellow, 1995

• NASA Fellow, 1995

Professional activities/recognition (e.g. consultancy, external examiner duties)

Executive Director of Future Genetics Limited

Managing Editor of Frontiers in Biosciences

Associate Editor of Frontiers in Immunology

Editorial board: Immunology

Regular reviewer of grant applications for the Wellcome Trust, Medical Research Council UK, Research funding agencies of Singapore, Germany, India, and others.

Publications

Journal Papers

(2012) Kishore, U. and Sim, RB., Factor H as a regulator of the classical pathway activation, Immunobiology 217 (2) : 162- 168

(2012) Kang, YH., Urban, BC., Sim, RB. and Kishore, U., Human complement Factor H modulates C1q-mediated phagocytosis of apoptotic cells., Immunobiology 217 (4) : 455- 464

(2012) Gupta, A., Kaul, A., Tsolaki, AG., Kishore, U. and Bhakta, S., Mycobacterium tuberculosis: Immune evasion, latency and reactivation, Immunobiology 217 (3) : 363- 374

(2012) Gupta, A., Kaul, A., Tsolaki, AG., Kishore, U. and Bhakta, S., Mycobacterium tuberculosis: Immune evasion, latency and reactivation, Immunobiology 217 (3) : 363- 374

(2011) Radanova, M., Vasilev, V., Deliyska, B., Kishore, U., Ikonomov, V. and Ivanova, D., Anti-C1q autoantibodies specific against the globular domain of the C1qB-chain from patient with lupus nephritis inhibit C1q binding to IgG and CRP, Immunobiology (In Press)

(2011) Nayak, A., Pedenekar, L., Reid, KB. and Kishore, U., Complement and non-complement activating functions of C1q: A prototypical innate immune molecule, Innate Immunity

(2011) Sudhakaran, C., Kishore, U., Anjana, RM., Unnikrishnan, R. and Mohan, V., Effectiveness of sitagliptin in asian Indian patients with type 2 diabetes-an Indian tertiary diabetes care center experience, Diabetes Technology & Therapeutics 13 (1) : 27- 32

(2011) Sumner, R., Parton, A., Nowicky, AV., Kishore, U. and Gidron, Y., Hemispheric lateralisation and immune function: A systematic review of human research, Journal of Neuroimmunology 240-241 1- 12

(2011) Sumner, RC., Parton, A., Nowicky, AV., Kishore, U. and Gidron, Y., Hemispheric lateralisation and immune function: A systematic review of human research, Journal of Neuroimmunology 240-241 1- 12

(2011) Nayak, A., Ansar, R., Verma, SK., Bonifati, DM. and Kishore, U., Huntington's disease: An immune perspective, Neurology Research International 2011 563784- Download publication

(2011) Tan, LA., Yang, AC., Kishore, U. and Sim, RB., Interactions of complement proteins C1q and factor H with lipid A and Escherichia coli: Further evidence that factor H regulates the classical complement pathway, Protein Cell 2 (4) : 320- 332

(2011) Verma, SK., Ganesan, TS., Kishore, U. and Parker, PJ., The tumor suppressor RASSF1A is a novel effector of small G protein Rap1A, Protein Cell 2 (3) : 237- 249

(2010) Tan, LA., Yu, B., Sim, FC., Kishore, U. and Sim, RB., Complement activation by phospholipids: The interplay of factor H and C1q, Protein & Cell 1 (11) : 1033- 1049

(2010) Al-Madani, WA., Siddiqi, NJ., Alhomida, AS., Khan, HA., Arif, IA. and Kishore, U., Increased urinary excretion of carnitine and acylcarnitine by mercuric chloride is reversed by 2,3-dimercapto-1-propanesulfonic acid in rats, International Journal of Toxicology 29 (3) : 313- 317

(2010) Madan, T., Reid, KB., Clark, H., Singh, M., Nayak, A., Sarma, PU., Hawgood, S. and Kishore, U., Susceptibility of mice genetically deficient in SP-A or SP-D gene to invasive pulmonary aspergillosis, Molecular Immunology 47 (10) : 1923- 1930

(2010) Nayak, A., Ferluga, J., Tsolaki, AG. and Kishore, U., The non-classical functions of the classical complement pathway recognition subcomponent C1q, Immunology Letters 131 (2) : 139- 150

(2009) Waters, P., Vaid, M., Kishore, U. and Madan, T., Lung surfactant proteins A and D as pattern recognition proteins, Advances in Experimental Medicine and Biology 653 74- 97

(2009) Shrive, AK., Martin, C., Burns, I., Paterson, JM., Martin, JD., Townsend, JP., Waters, P., Clark, HW., Kishore, U., Reid, KBM. and Greenhough, TJ., Structural characterisation of ligand-binding determinants in human lung surfactant protein D: Influence of Asp325, Journal of Molecular Biology 394 (4-10) : 776- 788 Download publication

(2009) Kishore, U., Target pattern recognition in innate immunity, Advances in Experimental Medicine and Biology 653 ix- x

(2009) Singh, M., Madan, T., Waters, P., Sonar, S., Singh, SK., Kamran, MF., Bernal, AL., Sarma, PU., Singh, VK., Crouch, EC. and Kishore, U., Therapeutic effects of recombinant forms of full-length and truncated human surfactant protein D in a murine model of invasive pulmonary aspergillosis, Molecular Immunology 46 (11-12) : 2363- 2369

(2008) Gadjeva, MG., Rouseva, MM., Zlatarova, AS., Reid, KBM., Kishore, U. and Kojouharova, MS., Interaction of human C1q with IgG and IgM: Revisited, Biochemistry 47 (49) : 13093- 13102

(2008) Roumenina, LT., Popov, KT., Bureeva, SV., Kojouharova, M., Gadjeva, M., Rabheru, S., Thakrar, R., Kaplun, A. and Kishore, U., Interaction of the globular domain of human C1q with Salmonella typhimurium lipopolysaccharide, Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 1784 (9) : 1271- 1276 Download publication

(2008) Mahajan, L., Madan, T., Kamal, N., Singh, VK., Sim, RB., Telang, SD., Ramchand, CN., Waters, P., Kishore, U. and Sarma, PU., Recombinant surfactant protein-D selectively increases apoptosis in eosinophils of allergic asthmatics and enhances uptake of apoptotic eosinophils by macrophages, International Immunology 20 (8) : 993- 1007

(2007) Ghai, R., Waters, P., Roumenina, LT., Gadjeva, M., Kojouharova, MS., Reid, KBM., Sim, RB. and Kishore, U., C1q and its growing family, Immunobiology 212 (4-5) : 253- 266

(2007) Sim, RB., Kishore, U., Villiers, CL., Marche, PN. and Mitchell, DA., C1q binding and complement activation by prions and amyloids, Immunobiology 212 (4-5) : 355- 362

(2007) Cordery, D., Kishore, U., Kyes, S., Shafi, MJ., Watkins, KR., Williams, TN., Marsh, K. and Urban, BC., Characterisation of Plasmodium falciparum macrophage migration inhibitory factor homologue, Journal of Infectious Diseases 195 (6) : 905- 912 Download publication

(2007) Roumenina, L., Bureeva, S., Kantardjiev, A., Karlinsky, D., Andia-Pravdivy, JE., Sim, R., Kaplun, A., Popov, M., Kishore, U. and Atanasov, B., Complement C1q-target proteins recognition is inhibited by electric moment effectors, Journal of Molecular Recognition 20 (5) : 405- 415

(2007) Tsacheva, I., Radanova, M., Todorova, N., Argirova, T. and Kishore, U., Detection of autoantibodies against the globular domain of human C1q in the sera of systemic lupus erythematosus patients, MOLECULAR IMMUNOLOGY 44 (8) : 2147- 2151

(2007) Bonifati, DM. and Kishore, U., Role of complement in neurodegeneration and neuroinflammation, Molecular Immunology 44 (5) : 999- 1010

(2006) Zlatarova, AS., Rouseva, M., Roumenina, LT., Gadjeva, M., Kolev, M., Dobrev, I., Olova, N., Ghai, R., Jensenius, JC., Reid, KBM., Kishore, U. and Kojouharova, MS., Existence of different but overlapping IgG- and IgM-binding sites on the globular domain of human C1q, Biochemistry 45 (33) : 9979- 9988

(2006) Roumenina, LT., Ruseva, MM., Zlatarova, A., Ghai, R., Kolev, M., Olova, N., Gadjeva, M., Agrawal, A., Bottazzi, B., Mantovani, A., Reid, KBM., Kishore, U. and Kojouharova, MS., Interaction of C1q with IgG1, C-reactive protein and pentraxin 3: Mutational studies using recombinant globular head modules of human C1q A, B, and C chains, Biochemistry 45 (13) : 4093- 4104

(2006) Kishore, U., Greenhough, TJ., Waters, P., Shrive, AK., Ghai, R., Kamran, MF., Bernal, AL., Reid, KB., Madan, T. and Chakraborty, T., Surfactant proteins SP-A and SP-D: Structure, function and receptors, Molecular Immunology 43 (9) : 1293- 1315

(2005) Roumenina, L., Zlatarova, A., Olova, N., Radanova, M., Kishore, U. and Kojouharova, M., Contribution of the C1q polypeptide chains in the recognition of CRP, Biotechnology and Biotechnological Equipment 19 (2 SUPPL. 2) : 122- 125

(2005) Singh, U., Nicholson, G., Urban, BC., Sargent, IL., Kishore, U. and Bernal, AL., Immunological properties of human decidual macrophages - A possible role in intrauterine immunity, Reproduction 129 (5) : 631- 637

(2005) Spreadbury, I., Kishore, U., Beeson, D. and Vincent, A., Inhibition of acetylcholine receptor function by seronegative myasthenia gravis non-IgG factor correlates with desensitisation, Journal of Neuroimmunology 162 (1-2) : 149- 156

(2005) Groeneveld, TWL., Oroszlan, M., Owens, RT., Faber-Krol, MC., Bakker, AC., Arlaud, GJ., McQuillan, DJ., Kishore, U., Daha, MR. and Roos, A., Interactions of the extracellular matrix proteoglycans decorin and biglycan with C1q and collectins, The Journal of Immunology 175 (7) : 4715- 4723

(2005) Roumenina, LT., Kantardjiev, AA., Atanasov, BP., Waters, P., Gadjeva, M., Reid, KBM., Mantovani, A., Kishore, U. and Kojouharova, MS., Role of Ca2+ in the electrostatic stability and the functional activity of the globular domain of human C1q, Biochemistry 44 (43) : 14097- 14109 Download publication

(2005) Madan, T., Kaur, S., Saxena, S., Singh, M., Kishore, U., Thiel, S., Reid, KB. and Sarma, PU., Role of collectins in innate immunity against aspergillosis, Med Mycol 43 Suppl 1 S155- S163

(2005) Kishore, U., Ghai, R., Greenhough, TJ., Shrive, AK., Bonifati, DM., Gadjeva, MG., Waters, P., Kojouharova, MS., Chakraborty, T. and Agrawal, A., Erratum to “Structural and functional anatomy of the globular domain of complement protein C1q” [Immunol. Lett. 95 (2) (2004) 113–128], Immunology Letters 101 (1) : 116- 116

(2005) Kishore, U., Bernal, AL., Kamran, MF., Saxena, S., Singh, M., Sarma, PU., Madan, T. and Chakraborty, T., Surfactant proteins SP-A and SP-D in human health and disease, Arch Immunol Ther Exp (Warsz) 53 (5) : 399- 417

(2005) Madan, T., Reid, KB., Singh, M., Sarma, PU. and Kishore, U., Susceptibility of mice genetically deficient in the surfactant protein (SP)-A or SP-D gene to pulmonary hypersensitivity induced by antigens and allergens of Aspergillus fumigatus, Journal of Immunology 174 (11) : 6943- 6954

(2004) Groeneveld, TWL., Seelen, MA., Duijs, JMGM., Bajema, IM., Prins, FA., Kishore, U., Salant, DJ., Verbeek, JS., Kooten, CV. and Daha, MR., Anti-C1q autoantibodies deposit in glomeruli but are only pathogenic in combination with glomerular C1q-containing immune complexes, Journal of Clinical Investigation 114 (5) : 679- 688

(2004) Kishore, U., Gaboriaud, C., Waters, P., Shrive, AK., Greenhough, TJ., Reid, KBM., Sim, RB. and Arlaud, GJ., C1q and tumor necrosis factor superfamily: Modularity and versatility, Trends in Immunology 25 (10) : 551- 561

(2004) McConville, J., Farrugia, ME., Beeson, D., Kishore, U., Metcalfe, R., Newsom-Davis, J. and Vincent, A., Detection and characterization of MuSK antibodies in seronegative myasthenia gravis, Annals of Neurology 55 (4) : 580- 584

(2004) Kojouharova, MS., Gadjeva, MG., Tsacheva, IG., Zlatarova, A., Roumenina, LT., Tchorbadjieva, MI., Atanasov, BP., Waters, P., Urban, BC., Sim, RB., Reid, KBM. and Kishore, U., Mutational analyses of the recombinant globular regions of human C1q A, B, and C chains suggest an essential role for arginine and histidine residues in the C1q-IgG interaction, The Journal of Immunology 172 (7) : 4351- 4358

(2004) Kishore, U., Ghai, R., Greenhough, TJ., Shrive, AK., Bonifati, DM., Gadjeva, MG., Waters, P., Kojouharova, MS., Chakraborty, T. and Agrawal, A., Structural and functional anatomy of the globular domain of complement protein C1q, Immunology Letters 95 (2) : 113- 128

(2003) Nauta, AJ., Bottazzi, B., Mantovani, A., Salvatori, G., Kishore, U., Schwaeble, WJ., Gingras, AR., Tzima, S., Vivanco, F., Egido, J., Tijsma, O., Hack, EC., Daha, MR. and Roos, A., Biochemical and functional characterization of the interaction between pentraxin 3 and C1q, European Journal of Immunology 33 (2) : 465- 473

(2003) Shrive, AK., Tharia, HA., Strong, P., Kishore, U., Burns, I., Rizkallah, PJ., Reid, KBM. and Greenhough, TJ., High-resolution structural insights into ligand binding and immune cell recognition by human lung surfactant protein D, Journal of Molecular Biology 331 (2) : 509- 523

(2003) Kojouharova, MS., Tsacheva, IG., Tchorbadjieva, MI., Reid, KBM. and Kishore, U., Localization of ligand-binding sites on human C1q globular head region using recombinant globular head fragments and single-chain antibodies, Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 1652 (1) : 64- 74

(2003) Kishore, U., Gupta, SK., Perdikoulis, MV., Kojouharova, MS., Urban, BC. and Reid, KBM., Modular organization of the carboxyl-terminal, globular head region of human C1q A, B, and C chains, Journal of Immunology 171 (2) : 812- 820

(2003) Singh, M., Madan, T., Waters, P., Parida, SK., Sarma, PU. and Kishore, U., Protective effects of a recombinant fragment of human surfactant protein D in a murine model of pulmonary hypersensitivity induced by dust mite allergens, Immunology Letters 86 (3) : 299- 307

(2003) Kishore, U., Zhang, W., Corlett, L., Waters, P., Glaichenhaus, N. and Willcox, N., Use of peptide: HLA class II complexes to study specific T cells in autoimmune myasthenia gravis, Annals of the New York Academy of Sciences 998 339- 342

(2002) Plested, CP., Tang, T., Spreadbury, I., Littleton, ET., Kishore, U. and Vincent, A., AChR phosphorylation and indirect inhibition of AChR function in seronegative MG, Neurology 59 (11) : 1682- 1688

(2002) Lu, JH., Teh, C., Kishore, U. and Reid, KBM., Collectins and ficolins: Sugar pattern recognition molecules of the mammalian innate immune system, BBA - Biochimica et Biophysica Acta 1572 (2-3) : 387- 400

(2002) Kishore, U., Kojouharova, MS. and Reid, KB., Recent progress in the understanding of the structure-function relationships of the globular head regions of C1q, Immunobiology 205 (4-5) : 355- 364

(2002) Kishore, U., Zhang, W., Corlett, L., Kelleher, A., Shiono, H. and Willcox, N., Use of peptide: HLA class II complexes to study specific T cells in myasthenia gravis, JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY 73 (2) : 229- 229

(2001) Kishore, U., Strong, P., Perdikoulis, MV. and Reid, KB., A recombinant homotrimer, composed of the alpha helical neck region of human surfactant protein D and C1q B chain globular domain, is an inhibitor of the classical complement pathway, The Journal of Immunology 166 (1) : 559- 565

(2001) Perdikoulis, MV., Kishore, U. and Reid, KB., Expression and characterisation of the thrombospondin type I repeats of human properdin, Biochimica et Biophysica Acta 1548 (2) : 265- 277

(2001) Madan, T., Kishore, U., Singh, M., Strong, P., Hussain, EM., Reid, KB. and Sarma, PU., Protective role of lung surfactant protein D in a murine model of invasive pulmonary aspergillosis, Infection and Immunity 69 (4) : 2728- 2731

(2001) Kishore, U. and Reid, KB., Structures and functions of mammalian collectins., Results Probl Cell Differ 33 225- 248 Download publication

(2001) Kishore, U., Madan, T., Singh, M. and Strong, P., Surfactant proteins A and D protect mice against pulmonary hypersensitivity induced by Aspergillus fumigatus antigens and allergens, Journal of Clinical Investigation 107 (4) : 467- 475

(2000) Kishore, U. and Reid, KB., C1q: structure, function, and receptors, Immunopharmacology 49 (1-2) : 159- 170

(2000) Aggarwal, NK., Kishore, U. and Agarwal, BB., Hanging-delayed death (a rare phenomenon), Medicine, Science and the Law 40 (3) : 270- 272

(2000) Madan, T., Singh, M., Saxena, S., Kishore, U., Strong, P., Reid, KBM. and Sarma, UP., Pulmonary surfactant protein A and D suppress eosinophilia and specific IgE antibodies in a murine model of allergic bronchopulmonary aspergillosis (ABPA), JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 105 (1) : S301- S301

(1999) Kishore, U. and Reid, KB., Modular organization of proteins containing C1q-like globular domain, Immunopharmacology 42 (1-3) : 15- 21

(1998) Kishore, U., Perdikoulis, MV., Strong, P. and Reid, KBM., A homotrimer of the B-chain globular head region of C1q is an inhibitor of C1q-mediated complement activation, MOLECULAR IMMUNOLOGY 35 (6-7) : 375- 375

(1998) Strong, P., Kishore, U., Morgan, C., Lopez Bernal, A., Singh, M. and Reid, KB., A novel method of purifying lung surfactant proteins A and D from the lung lavage of alveolar proteinosis patients and from pooled amniotic fluid, Journal of Immunological Methods 220 (1-2) : 139- 149

(1998) Leigh, LE., Ghebrehiwet, B., Perera, TP., Bird, IN., Strong, P., Kishore, U., Reid, KB. and Eggleton, P., C1q-mediated chemotaxis by human neutrophils: involvement of gClqR and G-protein signalling mechanisms, Biochemical Journal 330 (Pt 1) 247- 254

(1998) Perdikoulis, MV., Kishore, U. and Reid, KBM., Cloning, expression in E-coli and characterisation of human properdin TSRs., MOLECULAR IMMUNOLOGY 35 (6-7) : 374- 374

(1998) Kishore, U., Leigh, LE., Eggleton, P., Strong, P., Perdikoulis, MV., Willis, AC. and Reid, KB., Functional characterization of a recombinant form of the C-terminal, globular head region of the B-chain of human serum complement protein, C1q, Biochemical Journal 333 (Pt 1) 27- 32

(1998) Priyadarsiny, P., Madan, T., Kishore, U. and Sarma, PU., Role of lung surfactant proteins A & D in host defense against A-fumigatus antigens., JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 101 (1) : S78- S78

(1997) Madan, T., Eggleton, P., Kishore, U., Strong, P., Aggrawal, SS., Sarma, PU. and Reid, KB., Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages, Infection and Immunity 65 (8) : 3171- 3179

(1997) Eggleton, P., Reid, KB., Kishore, U. and Sontheimer, RD., Clinical relevance of calreticulin in systemic lupus erythematosus, Lupus 6 (7) : 564- 571

(1997) Madan, T., Kishore, U., Shah, A., Eggleton, P., Strong, P., Wang, JY., Aggrawal, SS., Sarma, PU. and Reid, KB., Lung surfactant proteins A and D can inhibit specific IgE binding to the allergens of Aspergillus fumigatus and block allergen-induced histamine release from human basophils, Clinical and Experimental Immunology 110 (2) : 241- 249

(1997) Kishore, U., Eggleton, P. and Reid, KB., Modular organization of carbohydrate recognition domains in animal lectins, Matrix Biology 15 (8-9) : 583- 592

(1997) Kishore, U., Sontheimer, RD., Sastry, KN., Zaner, KS., Zappi, EG., Hughes, GR., Khamashta, MA., Strong, P., Reid, KB. and Eggleton, P., Release of calreticulin from neutrophils may alter C1q-mediated immune functions, Biochemical Journal 322 (Pt 2) 543- 550

(1997) Kishore, U., Sontheimer, RD., Sastry, KN., Zappi, EG., Hughes, GR., Khamashta, MA., Reid, KB. and Eggleton, P., The systemic lupus erythematosus (SLE) disease autoantigen-calreticulin can inhibit C1q association with immune complexes, Clinical and Experimental Immunology 108 (2) : 181- 190

(1996) Wang, JY., Kishore, U., Lim, BL., Strong, P. and Reid, KB., Interaction of human lung surfactant proteins A and D with mite (Dermatophagoides pteronyssinus) allergens, Clinical and Experimental Immunology 106 (2) : 367- 373

(1996) Kishore, U., Wang, JY., Hoppe, HJ. and Reid, KB., The alpha-helical neck region of human lung surfactant protein D is essential for the binding of the carbohydrate recognition domains to lipopolysaccharides and phospholipids, Biochemical Journal 318 (Pt 2) 505- 511

(1996) Kishore, U., Wang, J-Y., Hoppe, H-J. and Reid, KBM., The α-helical neck region of human lung surfactant protein D is essential for the binding of the carbohydrate recognition domains to lipopolysaccharides and phospholipids, Biochemical Journal 318 (2) : 505- 511

(1995) Wang, JY., Kishore, U. and Reid, KB., A recombinant polypeptide, composed of the alpha-helical neck region and the carbohydrate recognition domain of conglutinin, self-associates to give a functionally intact homotrimer, FEBS Letters 376 (1-2) : 6- 10

(1994) Kishore, U., Chauhan, M., Choudhury, NR. and Khatri, GS., Overexpression of Escherichia coli single stranded DNA binding protein under bacteriophage T 7 promoter, Indian Journal of Biochemistry and Biophysics 31 (4) : 249- 253

Conference Papers

(2007) Gadjeva, M., Ruseva, M., Zlatarova, A., Reid, KBM., Kishore, U. and Kojouharova, M., GC1q-ligand interactions - A Ca2+ dependent two-step process, 11th European Meeting on Complement in Human Disease, MOLECULAR IMMUNOLOGY (44) : 3983- 3983

(2004) Trouw, LA., Seelen, MA., Groeneveld, TWL., Duijs, JMGJ., Bajema, I., Prins, F., Kishore, U., Salant, DJ., Verbeek, SJ., Van Kooten, C. and Daha, MR., Induction of murine lupus-nephritis with an anti-C1q monoclonal antibody, 20th International Complement Workshop, MOLECULAR IMMUNOLOGY (41) : 319- 320

(2006) Kang, Y., Kishore, U., Sim, RB. and Urban, BC., Interaction of human C1q and Factor H with apoptotic cells, 10th Meeting on Complement in Human Disease, MOLECULAR IMMUNOLOGY (43) : 144- 144

(2004) Gadjeva, M., Roumenina, L., Zlatarova, A., Tsacheva, I., Reid, KBM., Kishore, U. and Kojouharova, M., Molecular mechanisms of recognition between complement component C1q globular domain and its specific ligands, 20th International Complement Workshop, MOLECULAR IMMUNOLOGY (41) : 232- 232

(2010) Gadjeva, M., Zlatarova, A., Ruseva, M., Kishore, U. and Kojouharova, M., Mutational analysis of ligand-binding activities of recombinant gC1q heterotrimer, 23rd International National Complement Workshop, MOLECULAR IMMUNOLOGY (47) : 2260- 2260

(2002) Kishore, U., Madan, T., Sarma, PU., Singh, M., Urban, BC. and Reid, KBM., Protective roles of pulmonary surfactant proteins, SP-A and SP-D, against lung allergy and infection caused by Aspergillus fumigatus, Workshop on Early Phases of Complement Activation and Innate Immunity, IMMUNOBIOLOGY (205) : 610- 618

(2004) Mitchell, DA., Villiers, CL., Foster, N., Kishore, U. and Sim, RB., Proteomic profiling of complement-dendritic cell interactions, 20th International Complement Workshop, MOLECULAR IMMUNOLOGY (41) : 277- 277

(2010) Nayak, A., Waters, P., Gadjeva, M., Ferluga, J., Reid, KBM., Sim, RB., Tsolaki, A. and Kishore, U., Recombinant forms of monomeric and homotrimeric globular regions of human C1q as inhibitors of complement and microglia activation induced by beta-amyloid and familial dementia peptides, 23rd International National Complement Workshop, MOLECULAR IMMUNOLOGY (47) : 2284- 2285

(2006) Groeneveld, TWL., Oroszlan, M., Faber-Krol, MC., Bakker, AC., Owens, RT., McQuillan, DJ., Kishore, U., Daha, MR. and Roos, A., Regulation of the inflammatory functions of C1q by the matrix proteoglycan biglycan, 10th Meeting on Complement in Human Disease, MOLECULAR IMMUNOLOGY (43) : 152- 152

(2004) Zhang, W., Leite, I., Kishore, U., Water, P. and Willcox, N., Use of ELISPOTS to monitor cloning of AChR-specific T-cells from myasthenia gravis patients, 7th International Congress of the International-Society-of-Neuroimmunology, JOURNAL OF NEUROIMMUNOLOGY (154) : 150- 150

Book Chapters

(2011) Nayak, A., Kishore, U. and Bonifati, DM., Neurodegeneration and neuroinflammation: the active role of complement. In: Farooqi, AA. ed. Molecular Aspects of Neurodegeneration and Neuroprotection. Bentham Science Publishers (Forthcoming)

(2009) Waters, P., Vaid, M., Kishore, U. and Madan, T., SP-A and SP-D as innate immune molecules. In: Kishore, U. ed. Target Pattern Recognition in Innate Immunity. Texas, USA : Springer-Landes Bioscience

Books

(2009) Kishore, U., Target pattern recognition in innate immunity. Springer

Other publications

Books and special editions edited:

Target Pattern Recognition in Innate Immunity. 2009. Series: Advances in Experimental Medicine and Biology, Vol. 653: Landes Bioscience and Springer. ISBN: 978-1-4419-0900-8

Currently writing

Text book on Host-Pathogen Interaction (Wiley-Blackwell)

Currently editing:

1. Special Issue of Frontiers in Biosciences titled Pulmonary surfactant in human health and disease

2. Special Topic Edition on C1q family for Frontiers in Immunology

3. Microbial Pathogenesis: Infection and Immunity (Springer)