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Research area(s)

− Molecular cell biology

− Genomic stability

− DNA replication dynamics Escherichia coli

− CRISPR-Cas and the interaction of the CRISPR-Cas system with DNA replication and genome stability in E. coli.

− Investigations into conflicts between DNA replication and transcription in living cells at a single molecule level.

− Investigations into how the above systems contribute to shaping the architecture of bacterial chromosomes.

Research Interests

The Rudolph Lab is involved in a variety of active research areas. My long-standing interests in DNA replication and genomic stability have led me to focus on how these factors impact DNA repliation, chromosome dynamics and  chromosomal architecture. Currently we have a number of active projects:

− We investigate DNA replication dynamics in Escherichia coli with a particular focus on the termination aspect of DNA replication. We demonstrated for the first time that RecG helicase can process intermediates that form when replication complexes fuse. Our recent findings highlight that a surprising number of proteins act to process replication termination intermediates, thereby avoiding severe problems for cell-cycle progression and genome stability.

− We are investigating replication-transcription conflicts at a single molecule level in collaboration with Prof. Mark Leake (York University).

− In collaboration with Ed Bolt (University of Nottingham) we investigate the role of CRISPR-Cas systems on DNA replication and genome stability.

− We are investigating how all of the above system contribute towards shaping the overall architecture of bacterial chromosomes.

The research in my lab was supported by funds from the Leverhulme Trust, the Royal Society, and BBSRC and Brunel University. Our current work is supported by funds from the BBSRC.

Research grants and projects


Building CRISPR Immunity Systems - How is Invading DNA Captured?
Funder: Biotechnology & Biological Sciences Research Council
Duration: February 2020 - January 2023
Precision to the very end: what happens when two replication forks converge during termination?
Funder: Biotechnology and Biological Sciences Research Council
Duration: September 2016 - August 2019

Collaborative grant with York University