Repo-Man function in embryogenesis and differentiation

Repo-Man (CDCA2) is a Protein Phosphatase 1 (PP1) targeting subunit that has been shown to be an important regulator of cell division. Recent studied from our laboratory have also shown that that Repo-Man is important for the correct assembly and maintenance of heterochromatin by an epigenetic mechanism. Since genome-wide profiling of pluripotent cells and differentiated cells suggests global chromatin remodelling during differentiation which results in a progressive transition from a fairly open chromatin configuration to a more compact state, we want to address the importance of this complex during early embryogenesis using zebrafish as a model system.

The aim of the project is to knock-down CDCA2 in zebrafish and analyse the early development  by imaging and immunofluorescence staining.  The student will conduct both whole animal gene knock out or tissue-specific gen knock-out selecting the tissues where CDCA2 is highly expressed in adults. Moreover, the in vivo function of this complex will be investigating by generating specific mutants using the newly developed CRISPR/Cas9 mediated genome engineering.

How to apply

If you are interested in applying for the above PhD topic please follow the steps below:

1. Contact the supervisor by email or phone to discuss your interest and find out if you would be suitable. Supervisor details can be found on this topic page. The supervisor will guide you in developing the topic-specific research proposal, which will form part of your application.
2. Click on the 'Apply here' button on this page and you will be taken to the relevant PhD course page, where you can apply using an online application.
3. Complete the online application indicating your selected supervisor and include the research proposal for the topic you have selected.

Good luck!

This is a self funded topic

Brunel offers a number of funding options to research students that help cover the cost of their tuition fees, contribute to living expenses or both. See more information here: https://www.brunel.ac.uk/research/Research-degrees/Research-degree-funding. The UK Government is also offering Doctoral Student Loans for eligible students, and there is some funding available through the Research Councils. Many of our international students benefit from funding provided by their governments or employers. Brunel alumni enjoy tuition fee discounts of 15%.

Meet the Supervisor(s)

Paola Vagnarelli - Qualifications: - PhD in Genetics and Molecular Biology, University of Pavia, Italy  Degree in Biological Sciences, University of Pavia, Italy Professional experience - 1993-1996  Postdoctoral Research Fellow, Dipartimento di Genetica e Microbiologia Universita’ di Pavia 1997    Visiting Scientist MRC Human Genetics Unit, Edinburgh 1998- 2012 Postdoctoral Research Fellow Wellcome Trust Centre for Cell Biology Edinburgh (Prof WC Earnshaw) 2102    Lecturer School of Health Sciences and Social Care, Department of Biosciences, Brunel University London